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β2 Laminins Modulate Neuronal Phenotype in the Rat Retina

https://doi.org/10.1006/mcne.1997.0632Get rights and content

Abstract

The production of cell types in the vertebrate retina follows a stereotyped time course. We have focused on a component of the extracellular matrix that may guide this schedule: the laminin β2 chain. Here, we have asked directly whether heterotrimeric laminins containing the laminin β2 chain can promote the production of presumptive rod photoreceptors (“rods”) and have correlated changes in rod production with changes in the production of other cell types. In cultures in which few rods, but many Müller and bipolar cells, are produced, the production of rods can be enhanced sixfold and that of bipolar cells can be reduced by 66%, by exposing cells to a laminin β2-rich matrix. Substitution of a laminin β2-depleted matrix (created with antisense RNA) returns the density of rods and bipolar cells to control levels. These linked alterations in phenotype expression suggest that laminins may control the choice between rod photoreceptor and rod bipolar cell fates.

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