Elsevier

Neurobiology of Disease

Volume 8, Issue 1, February 2001, Pages 136-146
Neurobiology of Disease

Regular Article
Neurofilament-Immunoreactive Neurons Are Not Selectively Vulnerable in Alzheimer's Disease

https://doi.org/10.1006/nbdi.2000.0361Get rights and content

Abstract

Abnormal neurofilament protein distribution and phosphorylation contributes to the cytoskeletal pathology of Alzheimer's disease. Anatomical studies suggest that cortical neurons immunoreactive for nonphosphorylated 200-kDa neurofilament are most vulnerable. We repeated these studies in formalin-fixed temporal lobe tissue from five Alzheimer's disease cases with tissue volume loss compared to five controls without tissue loss. Immunohistochemistry for nonphosphorylated and phosphorylated forms of the neurofilament protein was counterstained for Nissl substance and immuno-positive and -negative pyramidal neurons quantified using areal fraction counts. Compared with controls, cases with Alzheimer's disease had similar numbers of neurons expressing the nonphosphorylated neurofilament protein, suggesting these neurons are largely spared by the disease process. In Alzheimer's disease there was a significant increase in neurons containing phosphorylated neurofilament and tau proteins and a decrease in neurons devoid of neurofilament protein. Our results challenge the theory that neurons containing 200 kDa neurofilament are selectively vulnerable in Alzheimer's disease.

References (34)

  • T. Yamada et al.

    Relative sparing in Parkinson's disease of substantia nigra dopamine neurons containing calbindin-D28K

    Brain Res.

    (1990)
  • H. Braak et al.

    Neuropathological staging of Alzheimer-related changes

    Acta Neuropathol.

    (1991)
  • L.C. Cork et al.

    Phosphorylated neurofilament antigens in neurofibrillary tangles in Alzheimer's disease

    J. Neuropath. Exp. Neurol.

    (1986)
  • S. Couillard-Despres et al.

    Protective effect of neurofilament heavy gene overexpression in motor neuron disease induced by mutant superoxide dismutase

    Proc. Natl. Acad. Sci. USA

    (1998)
  • A. Delacourte et al.

    The biochemical pathway of neurofibrillary degeneration in aging and Alzheimer's disease

    Neurology

    (1999)
  • T. Duong et al.

    Immunoreactivity patterns in neurofibrillary tangles of the inferior temporal cortex in Alzheimer's disease

    Mol. Chem. Neuropathol.

    (1994)
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