Regular Article
Cellular transformation by SV40 large T antigen: interaction with host proteins

https://doi.org/10.1006/scbi.2000.0342Get rights and content

Abstract

SV40 large T antigen (TAg) is a powerful oncoprotein capable of transforming a variety of cell types. The transforming activity of TAg is due in large part to its perturbation of the retinoblastoma (pRB) and p53 tumor suppressor proteins. In addition, TAg binds to several other cellular factors, including the transcriptional co-activators p300 and CBP, which may contribute to its transformation function. Several other features of TAg that appear to contribute to its full transformation potential are yet to be completely understood. Study of TAg therefore continues to provide new insights into the mechanism of cellular transformation.

References (80)

  • AJ Levine

    p53, the cellular gatekeeper for growth and division

    Cell

    (1997)
  • B Vogelstein et al.

    p53 function and dysfunction

    Cell

    (1992)
  • DP Lane et al.

    MDM2—arbiter of p53’s destruction

    Trends Biochem Sci

    (1997)
  • SR Grossman et al.

    p300/MDM2 complexes participate in MDM2-mediated p53 degradation

    Mol Cell

    (1998)
  • H Symonds et al.

    p53-dependent apoptosis suppresses tumor growth and progression in vivo

    Cell

    (1994)
  • H Zhong et al.

    Phosphorylation of NF-kappa B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300

    Mol Cell

    (1998)
  • ML Avantaggiati et al.

    Recruitment of p300/CBP in p53-dependent signal pathways

    Cell

    (1997)
  • SC Tsai et al.

    Simian virus 40 large T antigen binds a novel Bcl-2 homology domain 3-containing proapoptosis protein in the cytoplasm

    J Biol Chem

    (2000)
  • A Srinivasan et al.

    The large tumor antigen of simian virus 40 encodes at least two distinct transforming functions

    J Virol

    (1989)
  • TD Kierstead et al.

    Association of p53 binding and immortalization of primary C57BL/6 mouse embryo fibroblasts by using simian virus 40 T-antigen mutants bearing internal overlapping deletion mutations

    J Virol

    (1993)
  • J Zhu et al.

    Transformation of a continuous rat embryo fibroblast cell line requires three separate domains of simian virus 40 large T antigen

    J Virol

    (1992)
  • R Eckner et al.

    Association of p300 and CBP with simian virus 40 large T antigen

    Mol Cell Biol

    (1996)
  • NL Lill et al.

    p300 family members associate with the carboxyl terminus of simian virus 40 large tumor antigen

    J Virol

    (1997)
  • KS Campbell et al.

    DnaJ/hsp40 chaperone domain of SV40 large T antigen promotes efficient viral DNA replication

    Genes Dev

    (1997)
  • LJ Ko et al.

    p53: puzzle and paradigm

    Genes Dev

    (1996)
  • PW Rice et al.

    Efficient transcriptional activation of many simple modular promoters by simian virus 40 large T antigen

    J Virol

    (1993)
  • B Damania et al.

    TAF-like function of SV40 large T antigen

    Genes Dev

    (1996)
  • ML Avantaggiati et al.

    The SV40 large T antigen and adenovirus E1a oncoproteins interact with distinct isoforms of the transcriptional co-activator, p300

    EMBO J

    (1996)
  • SH Friend et al.

    A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma

    Nature

    (1986)
  • P Stiegler et al.

    The RB family of cell cycle regulatory factors

    J Cell Biochem

    (1998)
  • XQ Qin et al.

    Identification of a growth suppression domain within the retinoblastoma gene product

    Genes Dev

    (1992)
  • Y Tao et al.

    Subunit composition determines E2F DNA-binding site specificity

    Mol Cell Biol

    (1997)
  • J DeGregori et al.

    Cellular targets for activation by the E2F1 transcription factor include DNA synthesis- and G1/S-regulatory genes

    [published erratum appears in Mol Cell Biol 1995;

    (1995)
  • Mol Cell Biol, 15, 4215,...
  • DG Johnson et al.

    Expression of transcription factor E2F1 induces quiescent cells to enter S phase

    Nature

    (1993)
  • MC Blake et al.

    Transcription factor E2F is required for efficient expression of the hamster dihydrofolate reductase gene in vitro and in vivo

    Mol Cell Biol

    (1989)
  • QJ Hu et al.

    The regions of the retinoblastoma protein needed for binding to adenovirus E1A or SV40 large T antigen are common sites for mutations

    EMBO J

    (1990)
  • WG Jr Kaelin et al.

    Definition of the minimal simian virus 40 large T antigen- and adenovirus E1A-binding domain in the retinoblastoma gene product

    Mol Cell Biol

    (1990)
  • J Zalvide et al.

    The J domain of simian virus 40 large T antigen is required to functionally inactivate RB family proteins

    Mol Cell Biol

    (1998)
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    Corresponding author. Email: [email protected]

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