Abstract
Fusion of fluorescent probes to axonally transported proteins represents an established approach that enables live imaging of axonal transport. In this approach, in vivo examination of fluorescent particle dynamics provides information about the length, directionality, and the velocity by which axonally transported proteins travel along axons. Analysis of these parameters provides information about the distribution of axonal proteins and their dynamics in and between different subcellular compartments. Establishing the movement behavior of amyloid precursor protein within axons indicated that live imaging approaches offer the opportunity to significantly enhance our understanding of the biology as well as pathology of axonal transport. This chapter provides a fluorescence-based procedure for measuring axonal transport of APP in cultured newborn mouse hippocampal neurons.
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Acknowledgements
This work was supported by PICT-2008-0293 (ANPCyT)(T.L.F.), Alzheimer Association NIRG-10-172840 Grant (T.L.F.) and P3-0338 grant from the Slovenian Research Agency (G.B.S.).
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Falzone, T.L., Stokin, G.B. (2012). Imaging Amyloid Precursor Protein In Vivo: An Axonal Transport Assay. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 846. Humana Press. https://doi.org/10.1007/978-1-61779-536-7_25
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DOI: https://doi.org/10.1007/978-1-61779-536-7_25
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