Summary
The effects of capsaicin pretreatment of adult rats was investigated on consequences of unilateral paw inflammation induced by inoculation with Freund's adjuvant. Decrease in mechanical nociceptive threshold in the inflamed paw, as measured by the paw pressure test, was dose-dependently inhibited by capsaicin (20–150 mg/kg s.c.). In control rats, the antinociceptive action of morphine (0.8–1.9 mg/kg s. c.) was greater in the inflamed than in the non-inflamed paw; this difference was absent in capsaicin-treated animals. Increased volume or skin temperature of the inflamed paw was not influenced by capsaicin. It is concluded that capsaicin-sensitive, presumably C-fibre neurones, but not an alteration of the inflammation itself by capsaicin, mediate hyperalgesia and increased morphine antinociception in the rat paw with adjuvant-induced inflammation.
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References
Arvier PT, Chahl, LA, Ladd RJ (1977) Modification by capsaicin and compound 48/80 of dye leakage induced by irritants in the rat. Br J Pharmacol 59:61–68
Barthó L, Amann R, Saria A, Szolcsányi J, Lembeck F (1987) Peripheral effects of opioid drugs on capsaicin-sensitive neurones of the guinea-pig bronchus and rabbit ear. Naunyn-Schmiedeberg's Arch Pharmacol 336:316–320
Buck SH, Burks TF (1986) The neurophysiological effects of capsaicin: review of some recent observations. Pharmacol Rev 38:179–226
Colpaert FC, Donnerer J, Lembeck F (1983) Effects of capsaicin on inflammation and on the substance P content of nervous tissues in rats with adjuvant arthritis. Life Sci 32:1827–1834
Fitzgerald (1983) Capsaicin and sensory neurones — a review. Pain 15:109–130
Gamillscheg A, Holzer P, Donnerer J, Lembeck F (1984) Effect of neonatal treatment with capsaicin on carrageenan-induced paw oedema in the rat. Naunyn-Schmiedeberg's Arch Pharmacol 326:340–342
Haegerstrand A, Dalsgaard C-J, Jonsson C-E (1987) Effects of capsaicin pretreatment on the inflammatory response to scalding injury in the rat. Acta Physiol Scand 130:345–348
Hara A, Sakurada T, Sakurada S, Matsumura H, Kisara K (1984) Antinociceptive effects of neonatal capsaicin in rats with adjuvant arthritis. Naunyn-Schmiedeberg's Arch Pharmacol 326:248–253
Hargreaves K, Dubner R, Brown F, Flores C, Joris J (1988) A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain 23:77–88
Hayes AG, Tyers MB (1980) Effect of capsaicin on nociceptive heat, pressure and chemical thresholds and on substance P levels in the rat. Brain Res 189:561–564
Holzer P, Saria A, Skofitsch G, Lembeck F (1981) Increase in tissue concentrations of histamine and 5-hydroxytryptamine following capsaicin treatment of newborn rats. Life Sci 29:1099–1105
Jancsó G, Jancsó-Gabor A (1980) Effect of capsaicin on morphine analgesia — possible involvement of hypothalamic structures. Naunyn-Schmiedeberg's Arch Pharmacol 311:285–288
Jancsó G, Király E, Jancsó-Gabor A (1977) Pharmacologically-induced selective degeneration of chemosensitive primary sensory neurones. Nature 270:741–743
Jancsó G, Király E, Jancsó-Gábor A (1980) Chemosensitive pain fibres and inflammation. Int J Tissue React 2:57–66
Jancsó N, Jancsó-Gábor A, Szolcsanyi J (1967) Direct evidence for neurogenic inflammation and its prevention by denervation and pretreatment with capsaicin. Br J Pharmacol Chemother 31:138–151
Kayser V, Guilbaud G (1983) The analgesic effects of morphine, but not those of the enkephalinase inhibitor thiorphan, are enhanced in arthritis. Brain Res 267:131–138
Levine JD, Dardick SJ, Roizen MF, Helms C, Basbaum AI (1986) Contribution of sensory afferents and sympathetic efferents to joint injury in experimental arthritis. J Neurosci 6:3423–3429
Nagy JI (1983) Capsaicin: a chemical probe for sensory neuron mechanism. In: Iversen LL, Iversen SD, Snyder SH (eds) Handbook of Psychopharmacology. Plenum Press, New York, pp 185–235
Randall LO, Selitto JJ (1957) A method for measurement of analgesic activity on inflamed tissue. Arch Int Pharmacodyn 61:409–419
Saria A (1984) Substance P in sensory nerve fibres contributes to the development of oedema in the rat hind paw after thermal injury. Br J Pharmacol 82:217–222
Stein C, Millan MJ, Herz A (1988a) Unilateral inflammation of the hindpaw of rats as a model of prolonged noxious stimulation: alterations in behaviour and nociceptive thresholds. Pharmacol Biochem Behav 31:445–451
Stein C, Millan MJ, Yassouridis A, Herz A (1988b) Antinociceptive effects of mu- and kappa-agonists in inflammation are enhanced due to a peripheral opioid receptor-specific mechanism. Eur J Pharmacol 155:255–264
Stein C, Millan MJ, Shippenberg TS, Peter K, Herz A (1989) Peripheral opioid receptors mediating antinociception in inflammation. Evidence for involvement of mu-, delta- and kappareceptors. J Pharm Exp Ther 248:1269–1275
Stein C, Hassan AHS, Przewlocki R, Gramsch C, Peter K, Herz A (1990) Opioids from immunocytes interact with receptors on sensory nerves to inhibit nociception in inflammation. Proc Natl Acad Sci USA (in press)
Swingle KF, Grant TJ, Kwam DC (1971) Quantal responses in the Randall-Selitto assay. Proc Soc Exp Biol Med 137:536–538
Szolcsányi J (1985) Sensory receptors and the antinociceptive effects of capsaicin. In: Hakanson R, Sundler F (eds) Tachykinin antagonists. Elsevier, Amsterdam, pp 45–54
Szolcsányi J (1987) Selective responsiveness of polymodal nociceptors of rabbit ear to capsaicin, bradykinin and ultraviolet irradiation. J Physiol (Lond) 388:9–23
Szolcsányi J (1990) Capsaicin, irritation and desensitization. In: Green BG, Mason JR, Kare MR (eds) Chemical senses, vol 2: Irritation. Marcel Dekker, New York Basel, pp 141–168
Szolcsányi J, Anton F, Reeh PW, Handwerker HO (1988) Selective excitation by capsaicin of mechano-heat sensitive nociceptors in rat skin. Brain Res 446:262–268
van der Kooy D, Nagy JI (1985) Hyperalgesia mediated by peripheral opiate receptors in the rat. Behav Brain Res 17:203–211
Zimmermann M (1983) Guest editorial. Pain 16:109–110
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Barthó, L., Stein, C. & Herz, A. Involvement of capsaicin-sensitive neurones in hyperalgesia and enhanced opioid antinociception in inflammation. Naunyn-Schmiedeberg's Arch Pharmacol 342, 666–670 (1990). https://doi.org/10.1007/BF00175710
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DOI: https://doi.org/10.1007/BF00175710