Summary
The effects of the gamma-aminobutyric acid (GABA) antagonist bicuculline (BCC) on hindlimb motor performance was examined in mature spinal cats with spinal cord transections made either on the day of birth or at approximately two weeks postpartum and in chronic adult cats with spinal transections made in adulthood. In those adult operates, whose pre-drug performance was poor compared to neonatal operates, treadmill locomotion and weight support were improved dramatically by BCC administration. In neonatal operates (examined as adults), BCC administration increased the force of extension to abnormal levels and this increase appeared to disrupt locomotion. Immunocytochemical localization of GABA's synthetic enzyme, glutamic acid decarboxylase (GAD) within the spinal cords of these animals revealed an abnormal distribution of GAD reaction product only in newborn operates. The behavioral results indicate that the full extent of recovery in adult operates is prevented by inhibitory influences and this may contribute to the comparatively superior performance of neonatal operates i.e., the infant lesion effect. The anatomical results suggest that one requirement for the normal development of some intrinsic spinal circuitry is transneuronal regulation mediated by the maturation of descending systems.
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References
Albers WR, Brady RO (1958) The distribution of glutamic decarboxylase in the nervous system of the rhesus monkey. J Biol Chem 234: 926–928
Bard P (1938) Studies on the: cortical representation of somatic sensibility. Harvey Lectures 33: 143–169
Bregman BS, Goldberger ME (1983) Infant lesion effect. III. Anatomical correlates of sparing and recovery of function after spinal cord damage in newborn and adult cats. Dev Brain Res 9: 137–154
Christensen BN, Perl ER (1970) Spinal neurons specifically excited by noxious or thermal stimuli: marginal zone of the dorsal horn. J Neurophysiol 33: 293–307
Curtis DR, Duggan AW, Felix D, Johnston GA (1971) Bicuculline, an antagonist of GABA and synaptic transmission in the spinal cord of the cat. Brain Res 32: 69–96
Egger MD, Wall PD (1971) The plantar cushion reflex circuit: an oligosynaptic cutaneous reflex. J Physiol (Lond) 216: 483–501
Forssberg H, Grillner S (1973) The locomotion of the acute spinal cat injected with Clonidine i.v. Brain Res 50: 184–186
Forssberg H, Svartengren G (1983) Hardwired locomotor network in the cat revealed by a retained motor pattern to gastrocnemius after muscle transposition. Neurosci Lett 41: 283–288
Goldberger ME (1974) Recovery of movement after CNS lesions in monkeys. In: Stein D (ed) Recovery of function after neural lesions. Academic, New York, pp 265–317
Goldberger ME, Murray M (1978) Recovery of movement and axonal sprouting may obey some of the same laws. In: Cotman CW (ed) Neuronal plasticity. Raven, New York, pp 73–96
Goldberger ME (1983) Different patterns of recovery of motor function associated with different patterns of post lesion axonal growth. In: Seil C (ed) Nerve, organ and tissue regeneration: research perspectives. Academic, New York, pp 245–268
Goldberger ME, Coleman EP, Segal S (1985) Plasticity in the serotonin (5HT) system of the cat spinal cord. Anat Record 211: 70–71
Graham LT, Shank R, Werman R, Aprison MH (1967) Distribution of some synaptic transmitter suspects in cat spinal cord. J Neurochem 14: 465–472
Graham LT, Aprison MH (1969) Distribution of some enzymes associated with the metabolism of glutamate, aspartate, gamma-aminobutyrate and glutamine in cat spinal cord. J Neurochem 16: 559–566
Grillner S, Zangger P (1979) On the central generation of locomotion in the low spinal cat. Exp Brain Res 34: 241–261
Kawasaki K, Matsushita A (1982) Gabaergic influence on the crossed extensor reflex. Life Sci 30: 1625–1629
Killackey HP, Belford G, Ryugo R, Ryugo DK (1976) Anomalous organization of the thalamocortical projections consequent to vibrissae removal in the newborn rat and mouse. Brain Res 104: 309–315
LeVay S, Wiesel TN, Hubel DH (1980) The development of ocular dominance columns in normal and visually deprived monkeys. J Comp Neurol 191: 1–51
McLaughlin BJ, Barber R, Saito K, Roberts E, Wu JY (1975) Immunocytochemical localization of glutamate decarboxylase in the rat spinal cord. J Comp Neurol 164: 305–322
Mendell LM (1984) Modifiability of spinal synapses. Physiol Rev 64: 260–324
Miyata Y, Otsuka M (1975) Quantitative histochemistry of gamma-aminobutyric acid in the cat spinal cord with special reference to presynaptic inhibition. J Neurochem 25: 239–244
Oertel WH, Schmechel DE, Tappaz ML, Kopin IJ (1981) Production of a specific antiserum to rat brain glutamic acid decarboxylase by injection of an antigen-antibody complex. Neuroscience 6: 2589–2700
Patrick JT, McBride WJ, Feiten DL (1982) Distribution of glycine, GABA, aspartate and glutamate in the rat spinal cord. Brain Res Bull 10: 415–418
Reh T, Kalil K (1981) Development of the pyramidal tract in the hamster. I. A light microscopic study. J Comp Neurol 200: 55–67
Rexed B (1952) The cytoarchitectonic organization of the spinal cord in the cat. J Comp Neurol 96: 415–495
Rizzoli AA (1968) Distribution of glutamic acid, aspartic acid, gamma amino butyric acid and glycine in six areas of cat spinal cord before and after transection. Brain Res 11: 11–18
Robinson GA, Goldberger ME (1986) The development and recovery of motor function in spinal cats. I. The infant lesion effect. Exp Brain Res 62: 373–386
Rossignol S, Barbeau H, Julien C (1985) Locomotion of the adult spinal cat and its modification by monoaminergic agonists and antagonists. In: Goldberger ME et al. (eds) Development and plasticity of the mammalian spinal cord. Liviana Press (in press)
Shatz CJ, Stryker MP (1977) Ocular dominance in layer IV of the cat visual cortex and the effects of monocular deprivation. J Physiol (Lond) 281: 267–283
Smith DE (1974) The effect of deafferentation on the postnatal development of Clarke's nucleus in the kitten — a Golgi study. Brain Res 74: 119–130
Sprague JM (1966) Interaction of cortex and superior colliculus in mediation of visually guided behavior in the cat. Science 153: 1544–1547
Sternberger LA (1979) Immunocytochemistry. Wiley, New York
Tappaz ML, Zivin JA, Kopin IJ (1976) Intraspinal glutamic decarboxylase distribution after transection of the cord at the thoracic level. Brain Res 111: 220–223
Thor KB, Roppolo JR, deGroat WC (1983) Naloxone induced micturation in unanesthetized paraplegic cats. J Urology 129: 202–205
Valverde F (1967) Apical dendritic spines of the visual cortex and light deprivation in the mouse. Exp Brain Res 3: 337–352
Vigh B, Bigh-Teichmann I (1973) Comparative ultrastructure of the cerebrospinal fluid-contacting neurons. Int Rev Cytol 35: 189–251
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Robinson, G.A., Goldberger, M.E. The development and recovery of motor function in spinal cats. Exp Brain Res 62, 387–400 (1986). https://doi.org/10.1007/BF00238858
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DOI: https://doi.org/10.1007/BF00238858