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Histochemical demonstration of GABA-like immunoreactivity in cobalt labeled neuron individuals in the insect olfactory pathway

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Summary

Individual olfactory neurons in the antennal lobes of Periplaneta americana were investigated for their morphological and neurochemical properties by means of intracellular cobalt injection and indirect peroxidase-antiperoxidase immunohistochemistry. GABA-like immunoreactivity was demonstrated in many local interneurons but not in uniglomerular projection neurons.

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References

  • Boeckh J, Ernst KD (1978) Contribution of single unit analysis in insects to an understanding of olfactory function. J Comp Physiol A 161:549–565

    Google Scholar 

  • Boeckh J, Ernst KD, Polz H (1983) Convergence of inputs from different sense organs upon neurones of the central antennal pathway in insects. In: Horn E (ed) Multimodal convergences in sensory systems. Gustav Fischer, Stuttgart New York, pp 143–148

    Google Scholar 

  • Boeckh J, Ernst KD, Sass H, Waldow U (1984) Anatomical and physiological characteristics of individual neurones in the central antennal pathway of insects. J Insect Physiol 30:15–26

    Google Scholar 

  • Boer HH, Schott LPC, Veenstra JA, Reichelt D (1980) Immunocytochemical identification of neural elements in the central nervous system of a snail, some insects, a fish, and a mammal with an antiserum to the molluscan cardio-excitatory tetrapeptid FMRF-amide. Cell Tissue Res 213:21–27

    Google Scholar 

  • Burrows M, Boeckh J, Esslen J (1982) Physiological and morphological properties of interneurones in the deutocerebrum of male cockroaches which respond to female pheromone. J Comp Physiol 145:447–457

    Google Scholar 

  • Ernst KD, Boeckh J (1983) A neuroanatomical study on the organisation of the central anternnal pathways in insects. Cell Tissue Res 229:1–22

    Google Scholar 

  • Frontali N, Pierantoni R (1973) Autoradiographic localisation of 3H-GABA in the cockroach brain. Comp Biochem Physiol A 44:1369–1372

    Google Scholar 

  • Hoskins SG, Homberg U, Kingan TG, Christensen TA, Hildebrand JG (1986) Immunocytochemistry of GABA in the antennal lobes of the sphinx moth Manduca sexta. Cell Tissue Res 244:243–252

    Google Scholar 

  • Hoyle G (1977) Identified neurons and behavior of arthropods. Plenum Press, New York London

    Google Scholar 

  • Kraus D, Malun D, Waldow U, Boeckh J (1988) Tracts between the deuto- and the protocerebrum in male Periplaneta americana, and distribution, fine structure, and connections of terminals of identified deutocerebral projection neurons. In: Beiträge zur 16. Götinger Neurobiologentagung

  • Peters BH, Römer H, Marquart V (1986) Spatial segregation of synaptic inputs and outputs in a locust auditory interneuron. J Comp Neurol 254:34–50

    Google Scholar 

  • Reaves TA Jr, Hayword JN (1979) Intracellular dyemarked enkephalin neurons in the magnocellular preoptic nucleus of the goldfish hypothalamus. Proc Natl Acad Sci USA 76:6009–6011

    Google Scholar 

  • Sass H (1976) Zur nervösen Codierung von Geruchsreizen bei Periplaneta americana. J Comp Physiol 107:49–65

    Google Scholar 

  • Selsam P (1987) Duftcodierung, Morphologie und synaptische Verschaltung identifizierter Neurone im Deutocerebrum der Schabe. PhD thesis, University of Regensburg

  • Sternberger LA (1979) Immunocytochemistry. 2nd edn. John Wiley, New York

    Google Scholar 

  • Stewart WW (1978) Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphtalimide tracer. Cell 14:741–759

    Google Scholar 

  • Tyrer NM, Shaw MK, Altman JS (1980) Intensification of cobaltfilled neurons in sections (light and electron microscopy). In: Strausfeld NJ, Miller TA (eds) Neuroanatomical techniques, insect nervous system. Springer, New York Heidelberg Berlin, pp 429–446

    Google Scholar 

  • Voigt T (1986) Cholinergic amacrine cells in the rat retina. J Comp Neurol 248:19–35

    Google Scholar 

  • Waldow U (1975) Multimodale Neurone im Deutocerebrum von Periplaneta americana. J Comp Physiol 101:329–341

    Google Scholar 

  • Waldrop B, Christensen TA, Hildebrand JG (1987) GABA-mediated synaptic inhibition of projection neurons in the antennal lobes of the sphinx moth, Manduca sexta. J Comp Physiol A 161:23–32

    Google Scholar 

  • Watson AHD, Burrows M (1981) Input and output synapses on identified motor neurons of a locust revealed by the intracellular injection of horseradish peroxidase. Cell Tissue Res 215:325–332

    Google Scholar 

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Distler, P. Histochemical demonstration of GABA-like immunoreactivity in cobalt labeled neuron individuals in the insect olfactory pathway. Histochemistry 91, 245–249 (1989). https://doi.org/10.1007/BF00490139

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  • DOI: https://doi.org/10.1007/BF00490139

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