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Dopamine and noradrenaline transport into subcellular vesicles of the striatum

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Summary

Dopamine storing vesicles were isolated from the caudate nucleus of the pig by differential centrifugation and incubated at various temperatures. The spontaneous release of endogenous dopamine was temperature-dependent. Incubation with 14C-dopamine or 14C(±)-noradrenaline revealed that the vesicles were able to take up catecholamines by two different transport mechanisms; one was dependent on ATP, magnesium and temperature, the other one was independent of ATP and magnesium, and partially dependent on temperature. The Km of the ATP-magnesium-dependent uptake was 1.52×10−6 M for dopamine and 3.45×10−6 M for noradrenaline. Incubation with dopamine increased the dopamine content of the vesicles and diminished the endogenous dopamine by approximately 90%. Addition of ATP and magnesium further increased the dopamine content without influencing the per cent exchange between endogenous and exogenous dopamine. The dopamine uptake at 37°C in the presence of ATP and magnesium was of short duration because of the thermo-lability of the vesicles. (+)-amphetamine competitively inhibited the ATP-magnesium-dependent uptake of dopamine and noradrenaline. Amantadine and desipramine influenced neither the ATP-magnesium-dependent nor the ATP-magnesium-independent uptake of the catecholamines.

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Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie der Universität, Würzburg, Germany

    A. Philippu & J. Beyer

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  1. A. Philippu
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  2. J. Beyer
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This work was supported by the Deutsche Forschungsgemeinschaft and the Stiftung Volkswagenwerk.

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Philippu, A., Beyer, J. Dopamine and noradrenaline transport into subcellular vesicles of the striatum. Naunyn-Schmiedeberg's Arch. Pharmacol. 278, 387–402 (1973). https://doi.org/10.1007/BF00501482

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  • DOI: https://doi.org/10.1007/BF00501482

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