Summary
For intervals up to 15 days after transection of the rat spinal cord the level of noradrenaline (NA), dopamine (DA), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and tryptophan were studied above and below the lesions. In the upper part NA, DA and 5-HT increased continuously, while 5-HIAA increased during the first 3 to 5 days and then returned to its original level. In the lower part NA had disappeared almost completely after 15 days, DA after 9 days, 5-HT and 5-HIAA after 5 days. During the first day after transection 5-HT showed an increase in the lower part as did DA for the first 3 days. The different time course for DA and NA suggests that part of the spinal DA serves an independent non-precursor role.
Similar content being viewed by others
References
Andén, N.-E.: Distribution of monoamines and dihydroxyphenylalanine decarboxylase activity in the spinal cord. Acta physiol. scand. 64, 197–203 (1965).
Andén, N.-E., Bédard, P., Fuxe, K., Ungerstedt, U.: Early and selective increase in brain dopamine after axotomy. Experientia (Basel) 28, 300–301 (1972).
Andén, N.-E., Fuxe, K., Henning, M.: Mechanisms of noradrenaline and 5-hydroxytryptamine disappearance induced by α-methyl-DOPA and α-methyl-metatyrosine. Europ. J. Pharmacol. 8, 302–309 (1969).
Andén, N.-E., Häggendal, J., Magnusson, T., Rosengren, E.: The time course of the disappearance of noradrenaline and 5-hydroxytryptamine in the spinal cord after transection. Acta physiol. scand. 62, 115–118 (1964).
Andén, N.-E., Magnusson, T., Roos, B.-E., Werdinius, B.: 5-Hydroxyindoleacetic acid of rabbit spinal cord normally and after transection. Acta physiol. scand. 64, 193–196 (1965).
Atack, C. V.: The determination of dopamine by a modification of the dihydroxy-indole fluorimetric assay. Brit. J. Pharmacol (in press) (1973).
Atack, C. V., Magnusson, T.: Individual elution of noradrenaline (together with adrenaline), dopamine, 5-hydroxytryptamine and histamine from a single, strong cation exchange column by means of mineral acid-organic solvent mixtures. J. Pharm. Pharmacol. 22, 625–627 (1970).
Bédard, P., Carlsson, A., Lindqvist, M.: Effect of a transverse cerebral hemisection on 5-hydroxytryptamine metabolism in the rat brain. Naunyn-Schmiedeberg's Arch. Pharmacol. 272, 1–15 (1972)
Bertler, Å., Carlsson, A., Rosengren, E.: A method for the fluorimetric determination of adrenaline and noradrenaline in tissue. Acta physiol. scand. 44, 273–292 (1958).
Carlsson, A., Falck, B., Fuxe, K., Hillarp, N.-Å.: Cellular localization of monoamines in the spinal cord. Acta physiol. scand. 60, 112–119 (1964).
Carlsson, A., Kehr, W., Lindqvist, M., Magnusson, T., Atack, C. V.: Regulation of monoamine metabolism in the central nervous system. Pharmacol. Rev. 24, 371–384 (1972).
Carlsson, A., Lindqvist, M., Magnusson, T., Atack, C.: Effect of acute transection on the synthesis and turnover of 5-HT in the rat spinal cord. Naunyn-Schmiedeberg's Arch. Pharmacol. (in press) (1973).
Carlsson, A., Magnusson, T., Rosengren, E.: 5-Hydroxytryptamine of the spinal cord normally and after transection. Experientia (Basel) 19, 359 (1963).
Clineschmidt, B. V., Pierce, J. E., Lovenberg, W.: Tryptophan hydroxylase and serotonin in spinal cord and brain stem before and after chronic transection. J. Neurochem. 18, 1593–1596 (1971).
Costa, E., Neff, N. H.: Isotopic and non-isotopic measurements of the rate of catecholamine biosynthesis. In: Biochemistry and pharmacology of the basal ganglia, pp. 141–156. Ed. Costa, Coté and Yahr. Hewlett, N.Y.: Raven Press 1966.
Dahlström, A., Fuxe, K.: Evidence for the existence of monoamine neurons in the central nervous system. II. Experimentally induced changes in the intraneuronal amine levels of bulbospinal neuron systems. Acta physiol. scand. 64, Suppl. 247 (1965).
Dahlström, A., Häggendal, J.: On the possible relation between different pools of adrenergic transmitter and heterogeneity of amine storage granules in nerve terminals. Acta physiol. pol. 23, 3 Supl. 4, 67–79 (1972).
Dahlström, A., Häggendal, J., Atack, C. V.: Localization and transport of serotonin. In: Brain serotonin and behavior. Ed. J. Barchas and E. Usdin (in press). 1972.
Häggendal, C. J., Dahlström, A. B.: The transport and life-span of amine storage granules in bulbospinal noradrenaline neurons of the rat. J. Pharm. Pharmacol. 21, 55–57 (1969).
Iversen, I. I., Glowinski, J., Axelrod, J.: The uptake and storage of H3-norepinephrine in the reserpine-pretreated rat heart. J. Pharmacol. exp. Ther. 150, 173–183 (1965).
Kehr, W., Carlsson, A., Lindqvist, M.: A method for the determination of 3,4-dihydroxyphenylalanine (DOPA) in brain. Naunyn-Schmiedeberg's Arch. Pharmacol. 274, 273–280 (1972).
Korf, J., Valkenburg-Sikkema, T.: Fluorimetric determination of 5-hydroxyindoleacetic acid in human urine and cerebrospinal fluid. Clin. chim. Acta 26, 301–306 (1969).
Kuhar, M. J., Roth, R. H., Aghajanian, G. K.: Selective reduction of tryptophan hydroxylase activity in rat forebrain after midbrain raphe lesions. Brain Res. 35, 167–176 (1971).
Lindqvist, M.: Quantitative estimation of 5-hydroxy-3-indole acetic acid and 5-hydroxytryptophan in the brain following isolation by means of a strong cation exchange column. Acta pharmacol. (Kbh.) 29, 303–313 (1971).
Magnusson, T., Rosengren, E.: Catecholamines of the spinal cord normally and after transection. Experientia (Basel) 19, 229 (1963).
McGeer, E. G., McGeer, P. L.: Catecholamine content of spinal cord. Canad. J. Biochem. Physiol. 40, 1141–1151 (1962).
Olson, L., Fuxe, K.: On the projections from the locus coeruleus noradrenaline neurons: The cerebellar innervation. Brain Res. 28, 165–171 (1971).
Persson, T.: Central and peripheral catecholamine turnover studied by means of 3H-DOPA and 3H-tyrosine. Acta pharmacol. (Kbh.) 27, 397–409 (1969).
Pichler, H., Suko, J., Hertting, G.: Action of drugs upon the formation of tritiated water from dl-7-H3-noradrenaline in the rat. Biochem. Pharmacol. 17, 1329–1337 (1968).
Sedvall, G.: Noradrenaline storage in skeletal muscle. Acta physiol. scand. 60, 39–50 (1964).
Shibuya, T., Anderson, E. G.: The influence of chronic cord transection on the effects of 5-hydroxytryptophan, l-tryptophan and pargyline on spinal neuronal activity. J. Pharmacol. exp. Ther. 164, 185–190 (1968).
Snider, S., Almgren, O., Carlsson, A.: The occurrence and functional significance of dopamine in some peripheral adrenergic nerves of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. (in press) (1973).
Snider, S. R., Carlsson, A.: The adrenal dopamine as an indicator of adrenomedullary hormone biosynthesis. Naunyn-Schmiedeberg's Arch. Pharmacol. 275, 347–357 (1972).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Magnusson, T. Effect of chronic transection on dopamine, noradrenaline and 5-hydroxytryptamine in the rat spinal cord. Naunyn-Schmiedeberg's Arch. Pharmacol. 278, 13–22 (1973). https://doi.org/10.1007/BF00501859
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00501859