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Variation in ten lysosomal hydrolase enzyme activities in inbred mouse strains

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Abstract

Activities of 10 lysosomal hydrolase enzymes (β-hexosaminidase, β-galactosidase, α-galactosidase, α-mannosidase, β-mannosidase, α-l-fucosidase, β-glucuronidase, α-glucosidase, α-N-acetylgalactosaminidase, and acid phosphatase) were determined in eight organs (brain, liver, kidney, spleen, heart, skeletal muscle, lung, and testis) in males and females of six inbred mouse strains (C57 BL/6J, C3H/HeJ, DBA/2J, BALB/cJ, P/J, and 129/J). Examples of enzyme-specific variation, organ-specific variation, and enzyme- and organ-specific variation were found. New enzyme-specific variants with the features of systemic regulators for α-l-fucosidase and β-mannosidase were found. Known variants were detected. Organ-specific variants had some of the properties expected for a new class of genes affecting multiple enzymes: organ-specific regulators.

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This work was supported by grants from the National Institutes of Health (NS-15281 and NS-11766), the Muscular Dystrophy Association (H. Houston Merritt Clinical Center for Muscular Dystrophy and Related Diseases), and the March of Dimes Birth Defects Foundation and a generous gift from the Alexander Rapaport Foundation.

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Johnson, W.G., Hong, J.L. & Knights, S.M. Variation in ten lysosomal hydrolase enzyme activities in inbred mouse strains. Biochem Genet 24, 891–909 (1986). https://doi.org/10.1007/BF00554527

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  • DOI: https://doi.org/10.1007/BF00554527

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