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The increase in Cl permeation across the Deiters' neuron membrane by GABA on its cytoplasmic side is abolished by protein kinase C (PKC) activators

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Summary

  1. 1.

    Cl ion outward permeation across microdissected Deiters' neuron plasma membranes is augmented by GABA on the membrane cytoplasmic side. When these neurons are preincubated with a PKC activator, phorbol-12,13-dibutyrate (PdBu), there is a complex pattern of effects on basal and GABA-activated36Cl in → out permeation. A distinct fact is an increase in basal Cl passage and a disappearance of the 10−6 M GABA effect at [PdBu] = 0.1µM.

  2. 2.

    Likewise, 0.1µM oleylacetylglycerol (OAG) treatment erases the effect completely, further supporting a role for PKC in modulating GABA-stimulated Cl in → out permeation.

  3. 3.

    The inactive ester, phorbol-12,13-didecanoate (Pdd), at 0.1µM, does not affect GABA stimulation of Cl passage.

  4. 4.

    High concentrations (15-20µM) of OAG and PdBu block the “intracellular” GABA effect. However, the 20µM PdBu effect is reversed by 30µM H7.

  5. 5.

    These results indicate a role of endogenous PKC in Cl extrusion by GABAA receptors on the cytoplasmic side of the Deiters' neuron membrane.

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Rapallino, M.V., Cupello, A. & Hydén, H. The increase in Cl permeation across the Deiters' neuron membrane by GABA on its cytoplasmic side is abolished by protein kinase C (PKC) activators. Cell Mol Neurobiol 13, 547–558 (1993). https://doi.org/10.1007/BF00711463

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  • DOI: https://doi.org/10.1007/BF00711463

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