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GM1 ganglioside modulates prostaglandin E1 stimulated adenylyl cyclase in neuro-2A cells

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Abstract

This study demonstrates modulation by GM1 ganglioside of prostaglandin E1 (PGE1)-induced cAMP formation in Neuro-2a neuroblastoma cells. Pretreatment of the cells with neuraminidase, an enzyme that increases cell surface GM1, resulted in significant elevation of PGE1-induced cAMP formation, as did preincubation of the cells with nmolar concentrations of GM1. Pretreatment with brain ganglioside mixture lacking GM1 had no effect. Cholera toxin B subunit, a specific GM1-binding ligand, inhibited adenylyl cyclase. When the concentration of exogenous GM1 in which the cells were preincubated was increased from nmolar to μmolar levels there was a dose-responsive fall off in cAMP elevation, attributed to progressive inhibition of adenylyl cyclase by increasing GM1. These results are interpreted as indicating modulation of this PGE1 receptor in Neuro-2a cells by plasma membrane-localized GM1 in a structure-specific manner.

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Abbreviations

PGE1:

prostaglandin E1

Ctx B:

B subunit of cholera toxin

BBG:

bovine brain ganglioside mixture

DMEM:

Dulbecco's modified Eagle's medium

FBS:

fetal bovine serum

IBMX:

3-isobutyl-1-methylxanthine

N'ase:

neuraminidase

D-PBS:

Dulbecco's phosphate-buffered saline

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Authors and Affiliations

  1. Department of Neurosciences MSB-H506, New Jersey Medical School, UMDNJ, 185 South Orange Avenue, 07103, Newark, New Jersey, USA

    Gusheng Wu, Zi-Hua Lu & Robert W. Ledeen

Authors
  1. Gusheng Wu
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  2. Zi-Hua Lu
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  3. Robert W. Ledeen
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Wu, G., Lu, ZH. & Ledeen, R.W. GM1 ganglioside modulates prostaglandin E1 stimulated adenylyl cyclase in neuro-2A cells. Glycoconjugate J 13, 235–239 (1996). https://doi.org/10.1007/BF00731498

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  • DOI: https://doi.org/10.1007/BF00731498

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