Abstract
Patch-clamping studies with native outer mitochondrial membranes show a complex behavior. In the range of potentials in which the polarity of the pipette is positive, the behavior resembles that of VDAC incorporated into bilayers. Accordingly, there is a decrease in conductance with voltage. An involvement of VDAC is also supported by responses of the patches to the presence of polyanion or treatment with succinic anhydride, both of which affect VDAC. In contrast, in the negative range of potential, the conductance of the patches generally increases with the magnitude of the voltage. The increase in conductance shows a biphasic time course which is consistent with a model in which channels are first activated (first phase) and then assembled into larger high-conductance channels (second phase). A variety of experiments support the notion that an assembly takes place. The time course of the conductance increase is consistent with formation of the second-phase channels from 6±1 subunits.
Similar content being viewed by others
References
Belmonte, G., Cescatti, L., Ferrari, B., Nicolussi, T., Ropele, M., and Menestrina, G. (1987)Eur. Biophys. J. 14, 349–358.
Benz, R., Schmid, A., and Dihanich, M. (1989)J. Bioenerg. Biomembr. 21, 439–450.
Blachly-Dyson, E., Peng, S. Z., Colombini, M., and Forte, M. (1989)J. Bioenerg. Biomembr. 21, 471–483.
Boheim, G., Hanke, W., and Eibl, H. (1980)Proc. Natl. Acad. Sci. 77, 3403–3407.
Bowman, C. L., and Tedeschi, H. (1983)Biochim. Biophys. Acta 731, 261–266.
Catterall, W. A. (1988)Science 242, 50–61.
Colombini, M. (1987)J. Bioenerg. Biomembr. 19, 309–320.
De Pinto, V., Ludwig, O., Krause, J., Benz, R., and Palmieri, F. (1987)Biochim. Biophys. Acta 894, 109–119.
Freitag, H., Neupert, W., and Benz, R. (1982)Eur. J. Biochem. 123, 629–636.
Füssle, R., Bhakdi, S., Sziegoleit, A., Tranum-Jensen, J., Kranz, T., and Wellensiek, H. J. (1981)J. Cell. Biol. 91, 83–94.
Holden, M. J., and Colombini, M. (1988)FEBS Lett. 241, 105–109.
Ikigai, H., and Nakae, T. (1984)FEMS Lett. 24, 319–322.
Kinnally, K. W., Tedeschi, H., and Mannella, C. A. (1987)FEBS Lett. 226, 83–87.
Kinnally, K. W., Tedeschi, H., Mannella, C. A., and Frisch, H. L. (1989)Biophys. J. 55, 1205–1213.
Makowski, L., Caspar, D. L. D., Phillips, W. C., and Goodenough, D. A. (1977)J. Cell Biol. 74, 629–645.
Mangan, P. S., and Colombini, M. (1987)Proc. Natl. Acad. Sci. USA 84, 4896–4900.
Mannella, C. A. (1987)J. Bioenerg. Biomembr. 19, 329–340.
Tedeschi, H., Bowman, C. L., and Mannella, C. A. (1987)J. Membr. Biol. 97, 21–29.
Thieffry, M., Chich, J.-P., Goldschmidt, D., and Henry, J.-P. (1988)EMBO J. 7, 1449–1454.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tedeschi, H., Kinnally, K.W. & Mannella, C.A. Properties of channels in the mitochondrial outer membrane. J Bioenerg Biomembr 21, 451–459 (1989). https://doi.org/10.1007/BF00762517
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00762517