Summary
Three hormones were demonstrated in ultrathin sections of the rat thyroid using immunocytochemical methods with either a PAP complex or a protein A-gold complex as the tabel. In control rats, calcitonin was found to be present in all parafollicular cells and somatostatin in occasional cells. In rats pretreated with 5-hydroxytryptophan, serotonin was detected in all parafollicular cells as well. In serial ultrathin sections, the three hormones were seen to be localized in the same secretory granules.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K. &Watson, J. D. (1983)Molecular Biology of the Cell. pp. 717–765. New York, London: Garland.
Alumets, J., Hakanson, R., Lundqvist, G., Sundler, F. &Thorell, J. (1980) Ontogeny and ultrastructure of somatostatin and calcitonin cells in the thyroid gland of the rat.Cell Tissue Res. 206, 193–201.
Bendayan, M., Roth, J., Perrelet, A. &Orcl, L. (1980) Quantitative immunocytochemical localization of pancreatic secretory proteins in subcellular compartments of rat acinar cell.J. Histochem. Cytochem. 28, 149–60.
Bell, N. H. (1975) Further studies on the regulation of calcitonin releasein vitro.Horm. Metab. Res. 7, 77–83.
Buffa, R., Chayvialle, J. A., Fontana, P., Usellini, L., Capella, C. &Solcia, E. (1979) Parafollicular cells of rabbit thyroid store both calcitonin and somatostatin and resemble gut D cells ultrastructurally.Histochemistry 62, 281–8.
Ericson, L. E. (1970) Subcellular localization of 5-hydroxytryptamine in the parafollicular cells of mouse thyroid gland.J. Ultrastruct. Res. 31, 162–77.
Falck, B., Larson, B., Von Mecklenburg, C., Rosengren, E. &Svenaeus, K. (1964) On the presence of a second specific cell system in mammalian thyroid glandActa physiol. scand. 62, 491–2.
Falck, B. &Owman, Ch. (1968) 5-Hydroxytryptamine and related amines in endocrine cell systems.Adv. Pharmac. 6, 211–31.
Gillespie, J. S. (1980) Presynaptic receptors in the automatic nervous system. InAdrenergic Activators and Inhibitors (edited bySzekers, L.), pp. 352–426. Berlin: Springer-Verlag.
Hakanson, R., Owman, CH., Sporrong, B. &Sundler, F. (1971) Electron microscopic classification of amine-producing-endocrine cells by selective staining of ultrathin section.Histochemie 27, 226–42.
Hakanson, R., Melander, A., Owman, CH. &Sundler, F. (1973) Depletion of secretory granules, calcitonin, and formaldehyde-ozone-induced fluorescence from cat thyroid C cells by vitamin D2 treatment.Histochemie 36, 89–96.
Hargis, G. K., Williams, G. A., Reynolds, W. A., Chertow, B. S., Kukreja, S. C., Bowser, E. N. &Henderson, W. J. (1978) Effect of somatostatin on parathyroid hormone and calcitonin secretion.Endocrinology 102, 745–51.
Larsson, L. I. (1981) Peptide immunocytochemistry.Progress in Histochemistry and Cytochemistry 13, 4.
Linehan, W. M., Cooper, C. W., Bolman, R. M., &Wells, S. A. (1979) Inhibition ofin vivo secretion of calcitonin in the pig by somatostatin.Endocrinology 104, 1602–7.
Melander, A., Owman, CH. &Sundler, F. (1971) Effect of vitamin D2 stimulation on amine stores and secretory granules in the calcitonin cells of the mouse.Histochemie 25, 32–44.
Nunez, E. A. &Gershon, M. D. (1978) Cytophysiology of thyroid parafollicular cells.Int. Rev. Cytol. 52, 1–80.
Owman, CH., Hakanson, R. &Sundler, F. (1973) Occurrence and function of amines in endocrine cells producing polypeptide hormones.Fedn Proc. 32, 1785–91.
Roth, J., Ravazzola, M., Bendayan, M. &Orci, L. (1981) Application of the protein A A-gold technique for electron microscopic demonstration of polypeptide hormones.Endocrinology 108, 247–53.
Sternberger, L. A. (1979)Immunocytochemistry, 2nd edn. New York: John Wiley.
Sundler, F., Hakanson, R., Loren, I. &Lundquist, I. (1980) Amine storage and function in peptide hormone-producing cells.Invest. Cell Path. 3, 87–103.
Van Noorden, S. &Polak, J. M. (1983) Immunocytochemistry today: techniques and practice. InImmunocytochemistry (edited byPolak, J. M. andVan Noorden, S.), pp. 11–42. Bristol: Wright, PSG.
Van Noorden, S., Polak, J. M. &Pearse, A. G. E. (1977) Single cellular origin of somatostatin and calcitonin in the rat thyroid gland.Histochemistry 53, 243–7.
Zabel, M. (1979) Methodological examination and evaluation of specificity argentaffinic reaction in detection of enterochromaffic cells.Acta Med. Pol. 20, 479–82.
Zabel, M. (1982) Ultrastructural immunocytochemical localization of calcitonin in the C cells of the rat thyroid.Histochemistry 75, 419–24.
Zabel, M. (1983a) Ultrastructural localization of calcitonin in control and stimulated thyroid C cells of the rat using protein A-gold immunocytochemical technique.Histochemistry 77, 269–73.
Zabel, M. (1983b) Parafollicular cells of the rat thyroid gland after treatment with vitamin D3.Acta Anat. 118, 18–22.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zabel, M. Ultrastructural localization of calcitonin, somatostatin and serotonin in parafollicular cells of rat thyroid. Histochem J 16, 1265–1272 (1984). https://doi.org/10.1007/BF01003725
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01003725