Summary
Long standing questions related to brain mechanisms underlying perception can finally be resolved by direct visualization of the architecture and function of mammalian cortex. This advance has been accomplished with the aid of two optical imaging techniques with which one can literally see how the brain functions. The upbringing of this technology required a multi-disciplinary approach integrating brain research with organic chemistry, spectroscopy, biophysics, computer sciences, optics and image processing. Beyond the technological ramifications, recent research shed new light on cortical mechanisms underlying sensory perception. Clinical applications of this technology for precise mapping of the cortical surface of patients during neurosurgery have begun. Below is a brief summary of our own research and a description of the technical specifications of the two optical imaging techniques. Like every technique, optical imaging also suffers from severe limitations. Here we mostly emphasize some of its advantages relative to all alternative imaging techniques currently in use. The limitations are critically discussed in our recent reviews. For a series of other reviews, see Cohen (1989).
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References
Anglister, L., Farber, I.C., Shahar, A. and Grinvald, A. Localization of voltage-sensitive calcium channels along developing neurites; their possible role in regulating neurite elongation. Dev. Biol., 1982, 94: 351–365.
Bartfeld, E. and Grinvald, A. Architecture of processing modules in primate striate cortex underlying color, orientation and depth perception. Proc. Natl. Acad. Sci. USA., 1992, (in press).
Bonhoeffer, T. and Grinvald, A. Iso-orientation domains in cat visual cortex are arranged in pinwheel like patterns. Nature, 1991, 353: 429–431.
Cohen, L.B. Optical approaches to neuron function. Ann Rev. Physiol. 1989, 51: 487–582.
Frostig, R.D., Lieke, E.E., Ts'o, D.Y. and Grinvald, A. Cortical functional architecture and local coupling between neuronal activity and the microcirculation revealed by in vivo high resolution optical imaging of intrinsic signals. Proc. Natl. Acad. Sci. USA, 1990, 87: 6082–6086.
Grinvald, A., Salzberg, B.M. and Cohen, L.B. Simultaneous recording from several neurons in an invertebrate central nervous system. Nature, 1977, 268: 140–141.
Grinvald, A., Cohen, L.B., Lesher, S. and Boyle, M.B. Simultaneous optical monitoring of activity of many neurons in invertebrate ganglia using a 124 element photodiode array. J. Neurophysiol., 1981, 45: 829–840.
Grinvald, A. and Farber, I.C. Optical recording of calcium action potentials from growth cones of cultures neurons using a laser microbeam. Science, 1981, 212: 1164–1166.
Grinvald, A., Ross, W.N. and Farber, I.C. Simultaneous optical measurements of electrical activity from multiple sites on processes of cultured neurons. Proc. Natl. Acad. Sci. USA, 1981, 78: 3245–3249.
Grinvald, A., Hildesheim, R., Farber, I.C. and Anglister, L. Improved fluorescent probes for the measurements of rapid changes in membrane potential. Biophys. J., 1982, 39: 301–308.
Grinvald, A., Manker, A. and Segal, M. Visualization of the spread of electrical activity in rat hippocampal slices by voltage-sensitive optical probes. J. Physiol., 1982, 333: 269–291.
Grinvald, A., Fine, A., Farber, I.C. and Hildesheim, R. Fluorescence monitoring of electrical responses from small neurons and their processes. Biophys. J., 1983, 42: 195–198.
Grinvald, A. Real-time optical imaging of neuronal activity: from growth cones to the intact brain. Trends Neurosci., 1984, 7: 143–150.
Grinvald, A., Anglister, L., Freeman, J.A., Hildesheim, R. and Manker, A. Real-time optical imaging of naturally evoked electrical activity in intact frog brain. Nature, 1984, 308: 848–850.
Grinvald, A., Lieke, E., Frostig, R.D., Gilbert, C.D. and Wiesel T.N. Functional architecture of cortex revealed by optical imaging of intrinsic signals. Nature, 1986, 324: 361–364.
Grinvald, A., Salzberg, B.M., Lev-Ram, V. and Hildesheim, R. Optical recording of synaptic potentials from processes of single neurons using intracellular potentiometric dyes. Biophys. J., 1987, 51: 643–651.
Grinvald, A., Frostig, R.D., Lieke E. and Hildesheim, R. Optical imaging of neuronal activity. Physiol. Rev., 1988, 68: 1285–1366.
Grinvald, A., Bonhoeffer, T., Malonek, Shoham, Bartfeld, E., Arieli, A., Hildesheim R. and & Ratzlaff, E.H. Optical imaging of architecture and function in the living brain. In: Memory: Organization and Locus of Change. Oxford University Press, 1991: 49–85.
Grinvald, A., Frostig, R.D., Siegel, R.M. and Bartfeld, E. High-resolution optical imaging of functional brain architecture in the awake monkey. Proc. Natl. Acad Sci. USA, 1991, 88: 11559–11563.
Haugland, M.M., Ojemann, G.A. and Hochman, D.W. Optical imaging of epileptiform and functional activity in human cerebral cortex. Nature, 1992, 358: 668–671.
Kwong, K.K., Belliveau, J.W., Chesler, D.A., Goldberg, I.E., Weisskopf, R.M., Poncelet, B.P., Kennedy, D.N., Hoppel, B.E., Cohen, M.S., Turner, R., Cheng, H.-M., Brady, T.J. and Rosen, B.R. Dynamic magnetic resonance imaging of human brain activity during primary sensory stimulation. Proc. Natl. Acad. Sci. USA, 1992, 89: 5675–5679.
Lieke, E.E., Frostig, R.D., Arieli, A., Ts'o, D.Y., Hildesheim, R. and Grinald, A., Optical imaging of cortical activity: a realtime imaging using extrinsic dye-signals and high resolution imaging based on slow intrinsic-signals. Ann. Rev. Physiol., 1989, 51: 543–559.
Ogawa, S., Tank, D.W., Menon, R., Ellermann, J.M., Kim, S.-G., Merkle, H. and Ugurbil, K. Intrinsic signal changes accompanying sensory stimulation: functional brain mapping with magnetic resonance imaging. Proc. Natl. Acad. Sci. USA, 1992, 89: 5951–5955.
Orbach, H.S., Cohen, L.B. and Grinvald, A. Optical mapping of electrical activity in rat somatosensory and visual cortex. J. Neurosci., 1985, 5: 1886–1895.
Ratzlaff, E.H. and Grinvald, A. A tandem-lens epifluorescence macroscope: hunderd-fold brightness advantage for wide-field imaging. J. Neurosci. Methods, 1991, 36: 127–137.
Ts'o, D.Y., Frostig, R.D., Lieke, E.E. and Grinvald, A. Functional organization of primate visual cortex revealed by high resolution optical imaging. Science, 1990, 249: 417–420.
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Grinvald, A. Optical imaging of architecture and function in the living brain sheds new light on cortical mechanisms underlying visual perception. Brain Topogr 5, 71–75 (1992). https://doi.org/10.1007/BF01129033
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DOI: https://doi.org/10.1007/BF01129033