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A comparison of four techniques for mapping the distribution of serotonin and serotonin-containing neurons in fixed and living ganglia of the snail,Lymnaea

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Journal of Neurocytology

Summary

The distribution of serotonin and serotonin-containing neurons was studied in the ganglia of the CNS of the snailLymnaea stagnalis. Results of the application of three different labelling techniques on wholemount preparations were compared with each other and with the serotonin content of the ganglia, measured by high-performance liquid chromatography. Serotonin immunocytochemistry resulted in the highest number of labelled neurons, but the more recently developedin vivo method of 5,6- or 5,7-dihydroxytryptamine-induced pigmentation also proved to be a reliable technique for the visualization of serotonin-containing cell bodies. In comparison with these two techniques, the glyoxylic acid fluorescence method appeared to be less sensitive. The distribution and number of serotonin-containing neurons and biochemically measured serotonin in specific ganglia showed a close correlation. By combining the results of the three labelling techniques, a detailed map of serotonin-containing neurons was constructed, and this was compared with maps of identified neurons prepared from earlier electrophysiological studies. Previously described serotonergic neurons were consistently found, as well as several new serotonin-containirig cell types in the cerebral, visceral and parietal ganglia. A network of serotonin-containing inter- and intraganglionic axon tracts, and thin serotonergic fibres in the perineurium were also demonstrated. Thisin vivo andin vitro identification of serotonin-containing neurons will facilitate further neurophysiological analysis of serotonergic neural mechanisms inLymnaea.

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Authors and Affiliations

  1. Balaton Limnological Research Institute of the Hungarian Academy of Sciences, H-8237, Tihany, Hungary

    Kemenes, K. Elekes & L. Hiripi

  2. MRC Neurophysiology Research Group, School of Biology, University of Sussex, BN1 9QG, Falmer, Brighton, Sussex, UK

    Kemenes & P. R. Benjamin

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  1. Kemenes
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  2. K. Elekes
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  4. P. R. Benjamin
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Kemenes, Elekes, K., Hiripi, L. et al. A comparison of four techniques for mapping the distribution of serotonin and serotonin-containing neurons in fixed and living ganglia of the snail,Lymnaea . J Neurocytol 18, 193–208 (1989). https://doi.org/10.1007/BF01206662

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