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Opposite effects of rough and gentle handling with repeated saline administration on c-fos mRNA expression in the rat brain

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Summary

The rough handling with repeated saline administration (1.2 ml/kg s.c. for 7 days) enhanced cortical c-fos mRNA expression in the rat brain after a single saline stimulation (1.2 ml/kg s.c.) due to increasing baseline c-fos mRNA levels, whereas the gentle handling with repeated saline administration declined c-fos mRNA expression after a single injection due to decreasing the baseline of c-fos mRNA levels. These two types of handling with the repeated injection led to diametrically opposite results on c-fos mRNA expression after a single stimulation. Neither two types of handling with repeated saline injections affected the net increment of c-fos mRNA induction after a single stimulation, therefore, the effects of handling with repeated treatment on c-fos mRNA expression might be independent of the effects of a single saline stimulation. The present study suggests that c-fos mRNA induction after a single stimulation might be affected by the types or intensities of handling and that care must be taken to estimate c-fos mRNA induction.

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Asanuma, M., Ogawa, N., Hirata, H. et al. Opposite effects of rough and gentle handling with repeated saline administration on c-fos mRNA expression in the rat brain. J. Neural Transmission 90, 163–169 (1992). https://doi.org/10.1007/BF01250958

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  • DOI: https://doi.org/10.1007/BF01250958

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