Abstract
Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low expression levels are employed. Here, we discuss the advantages and restrictions of tagging recombinant proteins with histidines and purifying them by Ni2+-NTA chromatography.
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Abbreviations
- GST:
-
glutathione S-transferase
- NTA:
-
nitrilotriacetic acid
- His:
-
histidine
- PAGE:
-
polyacrylamide gel electrophoresis
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Schmitt, J., Hess, H. & Stunnenberg, H.G. Affinity purification of histidine-tagged proteins. Mol Biol Rep 18, 223–230 (1993). https://doi.org/10.1007/BF01674434
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DOI: https://doi.org/10.1007/BF01674434