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Long-term potentiation and olfactory memory formation in the carp (Cyprinus carpio L.) olfactory bulb

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Abstract

Long-term potentiation of synaptic transmission is considered to be an elementary process underlying the cellular mechanism of memory formation. In the present study we aimed to examine whether or not the dendrodendritic mitral-to-granule cell synapses in the carp olfactory bulb show plastic changes after their repeated activation. It was found that: (1) the dendrodendritic mitral-to-granule cell synapses showed three types of plasticity after tetanic electrical stimulation applied to the olfactory tract—long-term potentiation (potentiation lasting >1 h), short-term potentiation (potentiation lasting <1 h) and post-tetanic potentiation (potentiation lasting <10 min); (2) Long-term potentiation was generally induced when both the dendrodendritic mitral-to-granule cell synapses and centrifugal fiber-to-granule cell synapses were repeatedly and simultaneously activated; (3) long-term enhancement (>1 h) of the odor-evoked bulbar response accompanied the electrically-induced LTP, and; (4) repeated olfactory stimulation enhanced dendrodendritic mitral-to-granule cell transmission. Based on these results, it was proposed that long-term potentiation (as well as olfactory memory) occurs at the dendrodendritic mitral-to-granule cell synapses after strong and long-lasting depolarization of granule cells, which follows repeated and simultaneous synaptic activation of both the peripheral and deep dendrites (or somata).

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Abbreviations

C2 :

Component 2 wave

GC :

Granule cell

lMOT :

Lateral MOT

LOT :

Lateral olfactory tract

MC :

Mitral cell

MOT :

Medial olfactory tract

ON :

Olfactory nerve

OT :

Olfactory tract

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Acknowledgements

This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan and from the Ministry of Agriculture, Forestry and Fisheries of Japan. All experiments comply with the Animal Care and Use Committee guidelines of Yokohama City University and also with the regulations for the care and use of laboratory animals in Japan.

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Satou, M., Anzai, S. & Huruno, M. Long-term potentiation and olfactory memory formation in the carp (Cyprinus carpio L.) olfactory bulb. J Comp Physiol A 191, 421–434 (2005). https://doi.org/10.1007/s00359-005-0600-5

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