Abstract
Pericentrin (Pcnt) is a giant coiled-coil protein known to mediate microtubule organization. It has been recently reported that mitosis-specific centrosomal anchoring of γ tubulin complexes by Pcnt acts to control mitotic spindle organization, though little is known about the in vivo expression of Pcnt. In this study, we investigated Pcnt expression in mouse embryos. In situ hybridization analysis revealed preferential expression of Pcnt in quiescent G0 phase cells throughout the embryo with an unexpectedly low expression level in proliferating cells, suggesting that Pcnt might not play an important role in mitotic proliferation. Immunofluorescence analysis confirmed preferential expression of the Pcnt protein in G0 phase cells. Moreover, Pcnt was shown to be localized to the base of primary cilia in multiple embryonic tissues, in agreement with a recent study demonstrating the involvement of Pcnt in primary cilia formation using cultured mammalian cells.
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Acknowledgements
This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Communicated by B. G. Herrmann
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Miyoshi, K., Onishi, K., Asanuma, M. et al. Embryonic expression of pericentrin suggests universal roles in ciliogenesis. Dev Genes Evol 216, 537–542 (2006). https://doi.org/10.1007/s00427-006-0065-8
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DOI: https://doi.org/10.1007/s00427-006-0065-8