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The L-type calcium channel alpha 1C subunit gene undergoes extensive, uncoordinated alternative splicing

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Abstract

The α1C subunit is the pore-forming protein for the L-type calcium channel. Previous studies indicate that there is possible tissue-specific alternative splicing of this gene. In this study we cloned the entire open reading frame of the α1C subunit cDNA from adult rat cardiac myocytes in a single piece (6.64 kb). Using 75 positive clones that were identified by restriction enzyme mapping, we tested the alternative splicing patterns of the Cav1.2 gene that encodes the α1C subunit protein and focused on five loci: IS6, post-IS6, IIIS2, IVS3, and the c-terminus. The results indicate that: (1) alternative splicing occurs in most of the loci, giving rise to two or three different isoforms at those sites; (2) there is a predominant form for each splicing site, (3) there does not appear to be consistent coordination of splicing at multiple loci of this gene. Alternative splicing is not tissue-specific in most regions. (Mol Cell Biochem 269: 153–163, 2005)

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Authors and Affiliations

  1. Molecular and Cellular Cardiology, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan, USA

    Q. Ivy Fan, Kathleen M. Vanderpool, Hui-San Chung & James D. Marsh

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  1. Q. Ivy Fan
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  2. Kathleen M. Vanderpool
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  3. Hui-San Chung
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  4. James D. Marsh
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Correspondence to James D. Marsh.

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Fan, Q.I., Vanderpool, K.M., Chung, HS. et al. The L-type calcium channel alpha 1C subunit gene undergoes extensive, uncoordinated alternative splicing. Mol Cell Biochem 269, 153–163 (2005). https://doi.org/10.1007/s11010-005-3455-8

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  • DOI: https://doi.org/10.1007/s11010-005-3455-8

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