Abstract
The heterodimeric sweet taste receptors, T1R2 and T1R3, have recently been proposed to be associated with the brain glucose sensor. To identify whether sweet taste signaling is regulated in response to an ischemic injury inducing acute impairment of glucose metabolism, we investigated the spatiotemporal expression of the sweet taste receptors and their associated taste-specific G-protein α-gustducin in the rat hippocampus after ischemia. The expression profiles of both receptor subunits and α-gustducin shared overlapping expression patterns in sham-operated and ischemic hippocampi. Constitutive expression of both receptors and α-gustducin was localized in neurons of the pyramidal cell and granule cell layers, but their upregulation was detected in reactive astrocytes in ischemic hippocampi. Immunoblot analysis confirmed the immmunohistochemically determined temporal patterns of sweet-taste signaling proteins. These results suggest that the expression of sweet taste signaling proteins in astrocytes might be regulated in response to altered extracellular levels of glucose following an ischemic insult.
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Acknowledgments
This work was supported by Mid-career Researcher Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (No. 2010-0000333) and by a scholarship from Seoul Science Fellowship, Seoul City (Shin, Y-J).
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Mun-Yong Lee and Young Wha Moon contributed equally to this study.
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Shin, YJ., Park, JH., Choi, JS. et al. Enhanced Expression of the Sweet Taste Receptors and Alpha-gustducin in Reactive Astrocytes of the Rat Hippocampus Following Ischemic Injury. Neurochem Res 35, 1628–1634 (2010). https://doi.org/10.1007/s11064-010-0223-2
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DOI: https://doi.org/10.1007/s11064-010-0223-2