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SIRT1 Transcription Is Decreased in Visceral Adipose Tissue of Morbidly Obese Patients with Severe Hepatic Steatosis

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An Erratum to this article was published on 03 March 2010

Abstract

Background

Visceral adipose tissue is known to release greater amounts of adipokines and free fatty acids into the portal vein, being one of the most predictive factors of nonalcoholic fatty liver disease (NAFLD). Our study has the purpose to evaluate sirtuin 1 (SIRT1), adiponectin, Forkhead/winged helix (FOXO1), peroxisome proliferator-activated receptor (PPAR)γ1–3, and PPARβ/δ mRNA expression in morbidly obese patients in three different lipid depots: visceral (VAT), subcutaneous (SAT), and retroperitoneal (RAT). Recent studies suggest that SIRT1, a NAD+-dependent deacetylase, protects rats from NAFLD.

Methods

We divided the patients in two groups: those with slight or moderate steatosis (hepatic steatosis, HS) and other comprising individuals with severe steatosis associated or not with necroinflammation and fibrosis (severe hepatic steatosis, SHS). The adipose tissue depots were obtained during bariatric surgery. Total RNAs were extracted using TRIzol. The amount of genes of interest was determined by quantitative real-time polymerase chain reaction.

Results

When comparing the two groups of patients, a decrease in SIRT1 was observed in VAT of morbidly obese patients in SHS group (p = 0.006). The mRNA expression of the other genes showed no differences in VAT. No difference was found either in SAT or in RAT for all genes in the study. In addition, the homeostasis model assessment for insulin resistance (HOMA-IR) value was higher in SHS group compared to HS (p = 0.006). Also, our results show that the mRNA expression of SIRT1 and the value of HOMA-IR were positively correlated in VAT of SHS patients (r = 0.654; p = 0.048).

Conclusions

Downregulation of SIRT1 mRNA expression in VAT of SHS could be possible impairing mitochondria biogenesis and fatty acid oxidation, promoting severe steatosis in obese patients. Our results provide a possible proof of SIRT1 protective potential in VAT against NAFLD in humans.

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Abbreviations

NAFLD:

Nonalcoholic fatty liver disease

NASH:

Nonalcoholic steatohepatitis

AT:

Adipose tissue

FFA:

Free fatty acid

RAT:

Retroperitoneal adipose tissue

SAT:

Subcutaneous adipose tissue

VAT:

Visceral adipose tissue

FOXO1:

Forkhead/winged helix

SIRT1:

Sirtuin 1

PPAR:

Peroxisome proliferator-activated receptors

qRT-PCR:

Quantitative real-time polymerase chain reaction

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Acknowledgments

This work was supported by research grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Fundação de Apoio à Pesquisa do Rio Grande do Sul (FAPERGS). Dr. R. Margis is recipient of a research fellowship from the CNPq.

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Authors and Affiliations

  1. Departamento de Bioquímica, ICBS, UFRGS, Rua Ramiro Barcelos 2600—anexo, 90035-003, Porto Alegre, Rio Grande do Sul, Brazil

    Cíntia dos Santos Costa, Thais Ortiz Hammes, Francieli Rohden, Rogério Margis, Josiane Woutheres Bortolotto & Regina Maria Guaragna

  2. Centro de Biotecnologia, UFRGS, Porto Alegre, Rio Grande do Sul, Brazil

    Rogério Margis

  3. Centro de Obesidade e Síndrome Metabólica, Hospital São Lucas da PUCRS, Porto Alegre, Rio Grande do Sul, Brazil

    Alexandre Vontobel Padoin & Cláudio Cora Mottin

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  1. Cíntia dos Santos Costa
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  2. Thais Ortiz Hammes
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  8. Regina Maria Guaragna
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Correspondence to Regina Maria Guaragna.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s11695-010-0099-x

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Costa, C.d.S., Hammes, T.O., Rohden, F. et al. SIRT1 Transcription Is Decreased in Visceral Adipose Tissue of Morbidly Obese Patients with Severe Hepatic Steatosis. OBES SURG 20, 633–639 (2010). https://doi.org/10.1007/s11695-009-0052-z

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