Neurons switch from non-neuronal enolase to neuron-specific enolase during differentiation

doi:10.1016/0006-8993(80)91169-5

Brain Research

Volume 190, Issue 1, 19 May 1980, Pages 195-214

https://doi.org/10.1016/0006-8993(80)91169-5 Get rights and content

Abstract

The enolase (EC 4.2.1.11) isoenzymes, neuron-specific enolase (NSE,γγ) and non-neuronal enolase (NNE, αα), are markers for neurons and glia, respectively, in adult mammalian brain. In developing fetal and early postnatal brain, levels of non-neuronal enolase (NNE) are high. Neuron-specific enolase (NSE) appears only after neurogenesis begins in a given region and only slowly attains adult levels. Immunocytochemistry in developing rat and rhesus monkey brain reveals that proliferative zones that give rise to neurons are NNE(+). Thus, nerve cells must undergo a switch from NNE to NSE. In addition, study of neurons in cerebellum and neocortex reveals that they are NNE(+) during migration and only become NSE(+) in their final location, presumably after making full synaptic connections. Such migrating cells may contain hybrid enolase (άγ) and some (e.g. cerebellar stellate/basket cells) may not completely switch over to NSE even in the adult. Neuron-specific enolase is not only a specific molecular marker for mature nerve cells, but is closely correlated to the differentiated state.

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Neurons switch from non-neuronal enolase to neuron-specific enolase during differentiation

Abstract

Brain Research

Brain Research

Brain Research

Arch. Biochem. Biophys.

Brain Research

Brain Research

Biochem. biophys. Acta (Amst.)

Biochem. biophys. Res. Commun.

Brain Research

Nervous system specific proteins

J. Neurochem.

Demonstration of enolase activity connected to the brain specific protein 14-3-2

Scand. J. Immunol.

The nature of the two proteins of brain specific antigen 14-3-2

J. Neurochem.

Enolase: human tissue distribution and evidence for three different loci

Ann. hum. Genet.

Regional changes in CNS levels of the S-100 and 14-3-2 proteins during development and aging of the mouse

J. Neurochem.

Protein-A peroxidase: a valuable tool for the localization of antigens

J. Histochem. Cytochem.

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Develop. Biol.

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Anat. Embryol.

Histogenesis and morphogenesis of the central nervous system

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Biochemistry

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The developmental profile of neuronal and glial enolase in rat brain

Neurosci. Abstr.