Elsevier

Brain Research

Volume 348, Issue 2, 2 December 1985, Pages 391-396
Brain Research

Colocalization of [3H]muscimol and antisera to GABA and glutamic acid decar☐ylase within the same neurons in monkey retina

https://doi.org/10.1016/0006-8993(85)90464-0Get rights and content

Abstract

Two procedures have been used to test for the colocalization of different neuroanatomical γ-aminobutyric acid (GABA)ergic markers within the same neurons in primate retina. First, sequential immunocytochemical processing of sections was done using antisera to glutamic acid decar☐ylase and to GABA, and these antisera were visualized by peroxidase-antiperoxidase and fluorescein isothiocyanate techniques respectively. Colocalization of both antisera was found within the same neuron cell bodies. In the second experiment, immunocytochemical staining using GABA antiserum was performed on retinal tissue that had been previously incubated in vitro for neuronal uptake of [3H]muscimol. Both markers colocalized in 70% of the labeled cell body population.

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    • Cited by (0)

    This study was supported by USPHS Grants EY-01208 and EY-04536 from the National Institutes of Health and in part by RR-00166 to the Regional Primate Research Center at the University of Washington and an unrestricted grant from Research to Prevent Blindness, Inc.AA.H. is the recipient of a Dolly Green Scholar Award from Research to Prevent Blindness, Inc.

    **

    The authors gratefully acknowledge the technical help of Doris Ringer, Bradley Clifton and Ron Jones and the advice and assistance of P.V. Sarthy, Ph.D., regarding uptake studies.

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    Copyright © 1985 Published by Elsevier B.V.