Uptake of γ-aminobutyric acid andl-glutamic acid by synaptosomes from postmortem human cerebral cortex: multiple sites, sodium dependence and effect of tissue preparation
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2014, Neurochemistry InternationalCitation Excerpt :Our work and that of several other groups has shown that intact, metabolically active synaptosomes can be prepared from human brain obtained at autopsy that has been frozen and thawed; electron microscopic analysis showed that the preparations are morphologically comparable to those from fresh tissue (Hardy and Dodd, 1983). We optimized the preparation procedure (Dodd et al., 1986) and showed that the synaptosomes exhibit functions such as the uptake of transmitters against concentration gradients with appropriate kinetics (Dodd et al., 1989; Hardy et al., 1984) and stimulus-coupled Ca2+-dependent exocytotic transmitter release (Kuo and Dodd, 2011). Hahn et al. (2009) show electron micrographs of synaptosomes isolated from frozen human autopsy tissue together with immunoblots that demonstrate the enrichment of pre- and post-synaptic proteins including synaptophysin and PSD-95.
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1998, Progress in Brain ResearchSynaptosomal and brain slice cerebrocortical [<sup>3</sup>H]L-glutamate uptake in a rat model of chronic hepatic encephalopathy
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