Elsevier

Brain Research

Volume 578, Issues 1–2, 24 April 1992, Pages 61-68
Brain Research

Regional localization of the regulatory subunit (RIIβ) of the type II cAMP-dependent protein kinase in human brain

https://doi.org/10.1016/0006-8993(92)90230-7Get rights and content

Abstract

The distribution of the regulatory (RIIβ) subunits of type II cAMP-dependent protein kinase in cortical and subcortical areas was examined in human control and Alzheimer's disease (AD) brains. Four monoclonal antibodies generated against bovine brain RII, which crossreacted with human brain RIIβ, detected RII-immunoreactivity in pyramidal neurons of the hippocampus and frontal, occipital, parietal and superior temporal cortices and in non-pyramidal neurons of the amygdala and putamen. RIIβ immunoreactivity was localized to neuronal perikarya, proximal dendrites and cell processes. With the exception of rare processes in the ventroposterior lateral nucleus, RII-immunoreactivity was not seen in the thalamus. Other areas lacking RII-immunoreactivity included the midbrain, caudate nucleus and globus pallidus. RII-immunoreactivity was not detected in endothelia or glia. Except for the neocortex, the distribution of RIIβ immunoreactivity was the same in AD and non-demented control brains; however, cell bodies and their processes stained more intensely and uniformly in the neocortical regions of non-demented controls compared to AD. In the neocortex of AD, RIIβ immunoreactivity was substantially decreased in the superior temporal and occipital cortices, but not in the frontal cortex. Our data suggest that RII subunits are regionally distributed in the human brain. RII-immunoreactivity was decreased in some regions of neocortex in AD, but it did not preferentially colocalize with neurofibrillary tangles (NFT), senile plaques, or neuropil threads.

Reference (44)

  • BeebeS.J. et al.

    Molecular cloning of a tissue-specific protein kinase (C) from human testis - representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase

    Mol. Endocrinol.

    (1990)
  • BregmanD.B. et al.

    High affinity binding protein for the regulatory subunit of cAMP-dependent protein kinase II-B. Cloning, characterization, and expression of cDNAs for rat brain P150

    J. Biol. Chem.

    (1989)
  • CaddG. et al.

    Distinct patterns of cAMP-dependent protein kinase gene expression in mouse brain

    Neuron.

    (1989)
  • CleggC.H. et al.

    Genetic characterization of a brain-specific form of the type I regulatory subunit of cAMP-dependent protein kinase

  • ColeG. et al.

    Increased levels of protein kinase C in Alzheimer brain

    Brain Res.

    (1988)
  • DammermanM. et al.

    Sequence of a human MAP-2 sharing epitopes with Alzheimer neurofibrillary tangles

    J. Neurosci. Res.

    (1989)
  • DeCamilliP. et al.

    Heterogeneous distribution of the cAMP receptor protein RII in the nervous system: evidence for its intracellular accumulation on microtubules, microtubule-organizing centers, and in the area of the Golgi complex

    J. Cell Biol.

    (1986)
  • DudaiY.

    The cAMP cascade in the nervous system: molecular sites of action and possible relevance to neuronal plasticity

    CRC Crit. Rev. Biochem.

    (1987)
  • ErlichmanJ. et al.

    Identification of two subclasses of type II cAMP-dependent protein kinases: neural-specific and non-neural protein kinases

    J. Biol. Chem.

    (1980)
  • HymanB.T. et al.

    Alz-50 antibody recognizes Alzheimer-related neuronal changes

    Ann. Neurol.

    (1988)
  • IimotoD.S. et al.

    Aberrant casein kinase II in Alzheimer's disease

    Brain Res.

    (1989)
  • JahnsenT. et al.

    Molecular cloning, cDNA structure, and regulation of the regulatory subunit of type II cAMP-dependent protein kinase from rat ovarian granulosa cells

    J. Biol. Chem.

    (1986)
  • KatzmanR. et al.

    Advances in Alzheimer's disease

    FASEB J.

    (1991)
  • KhachaturianZ.S.

    Diagnosis of Alzheimer's disease

    Arch. Neurol.

    (1985)
  • KindlerS. et al.

    Molecular structure of microtubule-associated protein 2b and 2c from rat brain

    J. Biol. Chem.

    (1990)
  • LaemmliU.K.

    Cleavage of structural proteins during the assembly of the head of bacteriophage T4

    Nature

    (1970)
  • LeiserM. et al.

    Differential binding of the regulatory subunits (RII) of cAMP-dependent protein kinase II from bovine brain and muscle to RII-binding proteins

    J. Biol. Chem.

    (1986)
  • LewisS.A. et al.

    Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein

    Science

    (1988)
  • LohmannS.M. et al.

    High-affinity binding of the regulatory subunit (RII) of cAMP-dependent protein kinase to microtubule-associated and other cellular proteins

  • LudvigN. et al.

    Immunocytochemical localization of the neural-specific regulatory subunit of the type II cyclic AMP-dependent protein kinase to postsynaptic structures in the rat brain

    Brain Res.

    (1990)
  • LuoZ. et al.

    Identification of the MAP2- and P75-binding domain in the regulatory subunit (RIIβ) of type II cAMP-dependent protein kinase. Cloning and expression of the cDNA for bovine brain RIIβ

    J. Biol. Chem.

    (1990)

    • Cited by (0)

    View full text
    Copyright © 1992 Published by Elsevier B.V.