Expression of L1 and N-CAM cell adhesion molecules during development of the mouse olfactory system

doi:10.1016/0012-1606(89)90179-6

Developmental Biology

Volume 135, Issue 2, October 1989, Pages 272-286

https://doi.org/10.1016/0012-1606(89)90179-6 Get rights and content

Abstract

The expression of the neural adhesion molecules L1 and N-CAM has been studied in the embryonic and early postnatal olfactory system of the mouse in order to gain insight into the function of these molecules during development of a neural structure which retains neuronal turnover capacities throughout adulthood. N-CAM was slightly expressed and L1 was not significantly expressed in the olfactory placode on Embryonic Day 9, the earliest stage tested. Rather, N-CAM was strongly expressed in the mesenchyme underlying the olfactory placode. In the developing nasal pit, L1 and N-CAM were detectable in the developing olfactory epithelium, but not in regions developing into the respiratory epithelium. At early developmental stages, expression of the so-called embryonic form of N-CAM (E-N-CAM) coincides with the expression of N-CAM, whereas at later developmental stages and in the adult it is restricted to a smaller number of sensory cell bodies and axons, suggesting that the less adhesive embryonic form is characteristic of morphogenetically dynamic neuronal structures. Moreover, E-N-CAM is highly expressed at contact sites between olfactory axons and their target cells in the glomeruli of the olfactory bulb. L1 and N-CAM 180, the component of N-CAM that accumulates at cell contacts by interaction with the cytoskeleton are detectable as early as the first axons extend toward the primordial olfactory bulb. L1 remains prominent throughout development on axonal processes, both at contacts with other axons and with ensheathing cells. Contrary to N-CAM 180 which remains detectable on differentiating sensory neuronal cell bodies, L1 is only transiently expressed on these and is no longer detectable on primary olfactory neuronal cell bodies in the adult. Furthermore, whereas throughout development L1 has a molecular form similar to that seen in other parts of the developing and adult central nervous systems, N-CAM and, in particular, N-CAM 180 retain their highly sialylated form at least partially throughout all ages studied. These observations suggest that E-N-CAM and N-CAM 180 are characteristic of developmentally active structures and L1 may not only be involved in neurite outgrowth, but also in stabilization of contacts among fasciculating axons and between axons and ensheathing cells, as it has previously been found in the developing peripheral nervous system.

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Cell adhesion molecules (CAMs) that belong to the Ig superfamily (IgSF) have documented roles in axon outgrowth and navigation (Maness and Schachner, 2007; Plachez and Richards, 2005). In the olfactory system Ig-containing CAMs such as neural cell adhesion molecule (NCAM), olfactory cell adhesion molecule (OCAM) and L1 have been implicated in regulating olfactory development (Miragall et al., 1989; Treloar et al., 1997; Yoshihara et al., 1997). Therefore we selected IgSF8 for further study to test the hypothesis that it has a role in the development of the olfactory pathway.
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Full paperExpression of L1 and N-CAM cell adhesion molecules during development of the mouse olfactory system

Abstract

Neuroscience

Brain Res. Rev

Dev. Biol

Dev. Brain Res

Biochem. Biophys. Res. Commun

Dev. Brain Res

Dev. Brain Res

Brain Res

Dev. Biol

Brain Res

Dev. Brain Res

Differentiation

Dev. Biol

Trends Genet

J. Neuroimmunol

Dev. Brain Res

Immunohistochemical localization of the adhesion molecules L1, N-CAM and MAG in developing and adult optic nerve of mice

J. Comp. Neurol

Identification of the major proteins that promote neuronal process outgrowth on Schwann cells in vitro

J. Cell Biol

Ontogeny of Olfaction

Extension of neurites on axons is impaired by antibodies against specific neural cell surface glycoproteins

J. Cell Biol

Alterations in neural cell adhesion molecules during development of different regions of the nervous system

J. Neurosci

The development of the olfactory mucosa in the mouse: Light microscopy

J. Anat

The development of the olfactory mucosa in the mouse: Electron microscopy

J. Anat

Cell adhesion molecules in the regulation of animal form and tissue pattern

Annu. Rev. Cell Biol

Embryonic to adult conversion of neural cell adhesion molecules in normal and staggerer mice

Early epochal maps of two different cell adhesion molecules

The Cell in Contact

The neural cell adhesion molecule L1 is distinct from the N-CAM-related group of surface antigens BSP-2 and D2

EMBO J

Biosynthesis and membrane topography of the neural cell adhesion molecule L1

EMBO J

Development of the olfactory epithelium in the rat

Neuroblasts in the olfactory pits of mammals

Acta Anat. (Suppl)

An IgG monoclonal antibody to group B meningococci crossreacts with developmentally regulated polysialic acid units of glycoproteins in neural and extraneural tissues

J. Immunol

Neurite outgrowth patterns in cerebellar microexplant cultures are affected by antibodies to the cell surface glycoprotein L1

J. Neurosci

Full paper
Expression of L1 and N-CAM cell adhesion molecules during development of the mouse olfactory system