The rhombotin gene family encode related LIM-domain proteins whose differing expression suggests multiple roles in mouse development

doi:10.1016/0022-2836(92)90630-3

Journal of Molecular Biology

Volume 226, Issue 3, 5 August 1992, Pages 747-761

https://doi.org/10.1016/0022-2836(92)90630-3 Get rights and content

Abstract

The rhombotin (RBTN1 or Ttg-1) gene was first identified at a chromosome translocation in a T-cell acute leukaemia and later used to isolate two related genes (RBTN2 or Tig-2 and RBTN3). Complete characterization of these genes in man and mouse shows that all three encode cysteine-rich proteins with typical LIM domains. RBTN1 and RBTN3-derived proteins have 98% identity in the LIM domains but are located on separate chromosomes in man and in mouse while RBTN1 and RBTN2, both located on human chromosome Hp but are on separate chromosomes in mouse, are only 48% identical in this part of the protein. The exon organization of RBTN1 and RBTN3 genes are similar, both having an intron, absent from the RBTN2 gene, in the LIM2-encoding region. The remarkable similarity between rbtn-1 and rbtn-3 proteins is parallelled in their expression patterns in mouse development, since both genes show high expression in restricted areas of the brain, but little lymphoid expression, rbtn-2 expression, however, is more ubiquitous. This gene shows a low level of thymus expression but high expression in fetal liver, adult spleen and B-cell lines, consistent with a role in B-cell development. These results suggest multiple cellular targets for the action of these proteins during development.

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^☆: This research was supported, in part, by the Medical Research Council (U.K.) and by the National Cancer Institute. DHHS, under contract N01-CO-74101 with ABL.

^†: Present address: Klinikum der Albert-Ludwigs Universitat Freiburg, Abeteilung Innere Medizin I, Hugstetterstrasse 55, D-7800 Freiburg, Germany.

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Journal of Molecular Biology

ArticleThe rhombotin gene family encode related LIM-domain proteins whose differing expression suggests multiple roles in mouse development☆

Abstract

Genomics

Cell

Anal Biochem

Neuron

Cell

Cell

Blood

Cell

Genomics

Cell

Advan. Immunol

J. Mol. Biol

Genomics

Cell

Cell

Sensitive and high resolution in situ hybridisation to human chromosomes using biotin labelled probes: assignment of the human thymocyte CD1 antigen genes to chromosome 1

EMBO J

The gene SCL is expressed during early hematopoiesis and encodes a differentiation-related DNA-binding motif

Isolation of the human insulin-like growth factor genes: Insulin-like growth factor II and insulin genes are contiguous

Two distinct mechanisms for the SCL gene activation in the t(1:14) translocation of T-cell leukemias

Genes Chromosomes Cancer

The mechanism of chromosomal translocations t(11:14) involving the T cell receptor Cδ locus on human chromosome 14q11 and a transcribed region of chromosome 11p15

EMBO J

A cluster of chromosme 11p13 translocations found via distinct D-D and D-D-J rearrangements of the human T cell receptor δ chain gene

EMBO J

Alternating purine-pirimidine tracts may promote chromosomal translocations seen in a variety of human lymphoid tumours

EMBO J

The rhombotin gene belongs to a class of transcription regulators with a potential novel protein dimerisation motif

Oncogene

An unusual structure of a putative T cell oncogene which allows production of similar proteins from distinct mRNAs

EMBO J

The rhombotin family of cysteine-rich LIM-domain oncogenes: distinct members are involved in T-cell translocations to human chromosomes 11p15 and 11p13

A simple technique for generating probes for RNA in situ hybridisation: an adjunct to genome mapping exemplified by the RAG-1/RAG-2 gene cluster

Developmentally regulated and tissue specific expression of mRNAs encoding the two alternative forms of the LIM domain oncogene rhombotin: evidence for the thymus expression

Oncogene

Article
The rhombotin gene family encode related LIM-domain proteins whose differing expression suggests multiple roles in mouse development☆