[7] Purification of brain analogs of red blood cell membrane skeletal proteins: Ankyrin, protein 4.1 (synapsin), spectrin, and spectrin subunits
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Cited by (68)
Release of an ∼55 kDa fragment containing the actin-binding domain of β-spectrin by caspase-8 during FND-induced apoptosis depends on the presence of protein 4.1
2013, Archives of Biochemistry and BiophysicsCitation Excerpt :Vectashield® mounting medium with DAPI (1.5 μg/ml) was obtained from Vector Laboratories, Inc. (USA). Brain spectrin was isolated from pig brain tissue as described previously [17,18]. The purity of the protein was better than 95%, as tested by SDS–PAGE electrophoresis (7% gel) [19].
Lipid raft-dependent endocytosis of close homolog of adhesion molecule L1 (CHL1) promotes neuritogenesis
2012, Journal of Biological ChemistryCitation Excerpt :All steps were then performed at room temperature. Wells were blocked with 1% BSA in PBS for 1 h and incubated with purified mouse brain spectrin (6 μm) (17) for 1 h. Wells were washed and incubated for 1 h with increasing concentrations of CHL1-ID and L1-ID (15) diluted in PBS with 1% BSA. Plates were washed five times with PBS containing 0.05% Tween 20 (PBST) and incubated for 1 h with the monoclonal antibody 2C2 reacting with the cytoplasmic domains of L1 and CHL1 (15) diluted 1:5,000 in PBST containing 1% BSA.
Proteomics analysis identifies phosphorylation-dependent α-synuclein protein interactions
2008, Molecular and Cellular ProteomicsCitation Excerpt :The interaction of MAP1B with the carboxyl-terminal 45 amino acids of α-synuclein has been observed previously, although an increased affinity with phosphorylation was not previously recognized (26). A striking and novel observation in the pulldown using phosphorylated peptide was the prominence of non-erythrocyte αII and βII spectrins (fodrins) and the spectrin-interacting proteins ankyrin and band 4.1B (27, 28). In addition, we saw two isoforms of cytoplasmic actin, β- and γ-actin (29, 30); non-muscle myosins; and one species of neurofilament.
The 22.5 kDa spectrin-binding domain of ankyrinR binds spectrin with high affinity and changes the spectrin distribution in cells in vivo
2008, Protein Expression and PurificationBinding to the high-affinity M-type receptor for secreted phospholipases A<inf>2</inf> is not obligatory for the presynaptic neurotoxicity of ammodytoxin A
2006, BiochimieCitation Excerpt :Two weeks after the last booster dose the serum was collected and antibodies purified on a Protein A-Sepharose column (final protein concentration ~2 mg/ml). Specificity of the antibodies raised against the CTLD5 domain of the mouse M-type sPLA2R was tested by Western blot analysis of the bacterial inclusion bodies, and synaptic membrane fractions of porcine and mouse cerebral cortexes [42]. Three N-terminal fusion proteins of AtxA were prepared and characterized with the aim of observing the effects of the additional peptide.
Synapsin I is a major endogenous substrate for protein L-isoaspartyl methyltransferase in mammalian brain
2006, Journal of Biological Chemistry