Determination of dopamine norepinephrine, serotonin and their major metabolic products in rat brain by reverse-phase ion-pair high performance liquod chromatography with electrochemical detection
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Cited by (63)
Novel and sensitive reversed-phase high-pressure liquid chromatography method with electrochemical detection for the simultaneous and fast determination of eight biogenic amines and metabolites in human brain tissue
2014, Journal of Chromatography ACitation Excerpt :HPLC combined with ECD (HPLC-ECD) is still considered the method of choice given its potential as a specific and sensitive technique capable of automation, its low cost of analysis, and often simpler sample pretreatment requirements in comparison to for example HPLC with FD [5]. Many applications using ion-exchange reversed-phase HPLC with ECD (RP-HPLC-ECD) have been developed for the determination of these endogenous molecules in biological samples [6–11], but often only a limited number of compounds could be analysed in a single chromatographic run. We previously reported the development, optimization and validation of an ion-exchange reversed-phase HPLC with ECD method to determine eight biogenic amines and metabolites in mouse brain tissue [12].
Polymelamine modified edge plane pyrolytic graphite sensor for the electrochemical assay of serotonin
2014, TalantaCitation Excerpt :The determination of 5-HT is informative in the diagnosis of various diseases and hence makes it the subject of biologically and pharmacologically oriented research [23]. Several techniques have been employed for the determination of serotonin such as high performance liquid chromatography, capillary electrophoresis, mass and fluorescent spectrometry, and capillary liquid chromatography coupled with mass spectrometry [24–28]. However, most of these techniques require heavy and expensive instruments with sophisticated process, tedious time consuming pretreatment and derivatization.
Innovative green supercritical fluid chromatography development for the determination of polar compounds
2012, Journal of Chromatography ACitation Excerpt :Polar compounds have been widely studied by many chromatographic techniques. The most common method is reverse-phase liquid chromatography in conjunction with ion-pairing reagents to improve the retention of polar compounds [12–14]. These mobile phases contain at least one organic solvent, such as acetonitrile or methanol, and one ion-pairing agent such as trifluoroacetic acid (TFA), sodium octanesulfonate or hexanesulfonic acid.
Biogenic amines and their metabolites in mouse brain tissue: Development, optimization and validation of an analytical HPLC method
2010, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesCitation Excerpt :Also, a miniaturized LC system (column size: 150 mm × 1 mm, C18, 3 μm, flow rate: 0.05 ml/min) has replaced the conventional system (column size: 250 mm × 4.6 mm; C18, 5 or 10 μm, flow rate: 1 ml/min), yielding higher mass sensibility, requiring a small injection volume, significantly reducing mobile phase consumption rendering HPLC–ECD one of the most suitable methods for analysis of biogenic amines and metabolites [26]. Simultaneous determination of biogenic amines and their metabolites in brain tissue using HPLC–ECD, thought straightforward and feasible, seemed poorly achievable and limited to 2–5 compounds in previous studies [27–35]. This is most likely due to the difficulty in association of very early eluting of MHPG peak and very late eluting peak of 5-HT in the same chromatogram.