Cell

Cell

Volume 62, Issue 1, 13 July 1990, Pages 63-72
Journal home page for Cell

Article
Polarized sorting of viral glycoproteins to the axon and dendrites of hippocampal neurons in culture

https://doi.org/10.1016/0092-8674(90)90240-FGet rights and content

Abstract

Cultured hippocampal neurons were infected with a temperature-sensitive mutant of vesicular stomatitis virus (VSV) and a wild-type strain of the avian influenza fowl plague virus (FPV). The intracellular distribution of viral glycoproteins was monitored by immunofluorescence microscopy. In mature, fully polarized neurons the VSV glycoprotein (a basolateral protein in epithelial MDCK cells) moved from the Golgi complex to the dendritic domain, whereas the hemagglutinin protein of FPV (an apically sorted protein in MDCK cells) was targeted preferentially, but not exclusively, to the axon. The VSV glycoprotein appeared in clusters on the dendritic surface, while the hemagglutinin was distributed uniformly along the axonal membrane. Based on the finding that the same viral glycoproteins are sorted in a polarized fashion in both neuronal and epithelial cells, we propose that the molecular mechanisms of surface protein sorting share common features in the two cell types.

References (65)

  • A. Tixier-Vidal et al.

    Immunoelectron microscopy localization of synaptophysin in a Golgi subcompartment of developing hypothalamic neurons

    Neuroscience

    (1988)
  • R.D. Vale et al.

    Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility

    Cell

    (1985)
  • R.D. Vale et al.

    Kinesin: possible biological roles for a new microtubule motor

    Trends Biochem. Sci.

    (1986)
  • B. Wiedenmann et al.

    Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicle

    Cell

    (1985)
  • K.J. Angelides et al.

    Distribution and lateral mobility of voltage-dependent sodium channels in neurons

    J. Cell Biol.

    (1988)
  • P.W. Baas et al.

    Polarity orientation of microtubules in hippocampal neurons: uniformity in the axon and nonuniformity in the dendrite

  • G. Banker

    Trophic interactions between glial cells and hippocampal neurons in culture

    Science

    (1980)
  • G.A. Banker et al.

    Hippocampal neurons generate natural shapes in cell culture

  • W.O. Barlett et al.

    An electron microscopy study of the development of axons and dendrites by hippocampal neurons in culture. I. Cells which develop without intercellular contacts

    J. Neurosci.

    (1984)
  • W.O. Barlett et al.

    An electron microscopy study of the development of axons and dendrites by hippocampal neurons in culture. II. Synaptic relationships

    J. Neurosci.

    (1984)
  • J.R. Bartles et al.

    Biogenesis of the rat hepatocyte plasma membrane in vivo: comparison of the pathways taken by apical and basolateral proteins using subcellular fractionation

    J. Cell Biol.

    (1987)
  • M.K. Bennett et al.

    Release of putative exocytic vesicles from perforated MDCK cells

    EMBO J.

    (1988)
  • J.E. Bergmann et al.

    Immunoelectron microscopic studies of the intracellular transport of the membrane glycoprotein (G) of vesicular stomatitis virus in infected Chinese hamster ovary cells

    J. Cell Biol.

    (1983)
  • J.E. Bergmann et al.

    Passage of an integral membrane protein, the vesicular stomatitis virus glycoprotein, through the Golgi apparatus en route to the plasma membrane

  • J.E. Bergmann et al.

    Membrane insertion at the leading edge of motile fibroblasts

  • J.E. Bottestein et al.

    Growth of a rat neuroblastoma cell line in serum-free supplemented medium

  • A. Caceres et al.

    Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture

    J. Neurosci.

    (1986)
  • P. De Camilli et al.

    Heterogeneous distribution of the cAMP receptor protein RII in the nervous system: evidence for its intracellular accumulation on microtubules, microtubule-organizing center, and in the area of the Golgi complex

    J. Cell Biol.

    (1986)
  • C.G. Dotti et al.

    Experimetally induced alteration in the polarity of developing neurons

    Nature

    (1987)
  • C.G. Dotti et al.

    The establishment of polarity by hippocampal neurons in culture

    J. Neurosci.

    (1988)
  • D. Drenckhahn et al.

    Colocalization of band 3 with ankyrin and spectrin at the basal membrane of intercalated cells in the rat kidney

    Science

    (1985)
  • M. Dubois-Dalcq et al.

    Acute and persistent viral infections of differentiated nerve cells

    Rev, Infect. Dis.

    (1982)

    • Cited by (0)

    View full text
    Copyright © 1990