Ligand-induced redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulum

doi:10.1016/0092-8674(92)90476-S

Cell

Volume 68, Issue 2, 24 January 1992, Pages 353-364

https://doi.org/10.1016/0092-8674(92)90476-S Get rights and content

Abstract

Resident luminal endoplasmic reticulum (ER) proteins carry a targeting signal (usually KDEL in animal cells) that allows their retrieval from later stages of the secretory pathway. In yeast, the receptor that promotes this selective retrograde transport has been identified as the product of the ERD2 gene. We describe here the properties of a human homolog of this protein (hERD2). Overproduction of hERD2 improves retention of a protein with a weakly recognized variant signal (DDEL). Moreover, overexpression of KDEL or DDEL ligands causes a redistribution of hERD2 from the Golgi apparatus to the ER. Mutation of hERD2 alters the ligand specificity of this effect, implying that it interacts directly with the retained proteins. Ligand control of receptor movement may limit retrograde flow and thus minimize fruitless recycling of secretory proteins.

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ArticleLigand-induced redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulum

Abstract

Cell

Cell

J. Biol. Chem.

J. Mol. Biol.

Meth. Enzymol.

Cell

Cell

Cell

J. Mol. Biol.

J. Biol. Chem.

Cell

Trends Biochem. Sci.

Cell

J. Biol. Chem.

Distribution of receptors for transferrin and low density lipoprotein on the surface of giant HeLa cells

Distribution of ferritin receptors and coated pits on giant HeLa cells

EMBO J.

Sequences in the polyomavirus DNA regulatory origin involved in viral DNA replication and early gene expression

J. Virol.

Article
Ligand-induced redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulum