Research paperWhole cell recording from neurons in slices of reptilian and mammalian cerebral cortex
References (15)
Overestimation of the electrical length of neuron dendrites and synaptic electrotonic attenuation
Neurosci. Lett.
(1988)The use of the patch clamp technique to study second messenger-mediated cellular events
Neurosciences
(1988)- et al.
Guidance of pioneer growth cones: filopodial contacts and coupling revealed with an antibody to Lucifer Yellow
Dev. Biol.
(1982) - et al.
Gated currents generate single spike activity in amacrine cells of the tiger salamander retina
- et al.
Electrophysiological characterization of remote chemical synapses
J. Neurophysiol.
(1982) - et al.
Measurement of passive membrane parameters with whole-cell recordings from neurons in the intact amphibian retina
J. Neurophysiol.
(1989) - et al.
Cellular physiology of the turtle visual cortex: Distinctive properties of pyramidal and stellate neurons
J. Neurosci.
(1986)
Cited by (845)
Assessing Local and Branch-specific Activity in Dendrites
2022, NeuroscienceAdvances in the automation of whole-cell patch clamp technology
2019, Journal of Neuroscience MethodsCitation Excerpt :However, since proteolytic enzymes may damage the proteins on the cell membrane of interest, different approaches were sought and developed. To enable direct contact between the tip of a patch pipette and the tissue-covered cell membrane, these approaches implemented either a two-step process in which a separate, “cleaning” pipette was first used to remove the part of the tissue covering the cell body of interest (Edwards et al., 1989) or a one-step process in which positive pressure was applied to a patch pipette as it was penetrating the tissue and approaching the cell membrane (Blanton et al., 1989). The integration of differential interference contrast (DIC) optics was another major advancement for enabling patch clamping in brain slices, as the improved imaging quality offered by DIC-based microscopy enabled visually-guided patching of soma as well as dendrites of targeted neurons in mammalian brain slices (Stuart et al., 1993).