Short communication
MAP2 is localized to the dendrites of hippocampal neurons which develop in culture

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Abstract

The distribution of the microtubule-associated protein MAP2 in cultured hippocampal neurons was studied using immunocytochemistry with monoclonal antibodies. MAP2 was preferentially localized to dendritic, but not axonal, processes even in single isolated cells which developed without making intercellular contacts. Hence regional differences in the molecular composition of the neuronal cytoskeleton can develop independently of cell interactions. The presence of MAP2 may be a useful marker for identifying dendrites in cell culture.

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    This work was supported by NIH Grants NS17112 to G.B.) and N.S12333 (to O.S.) and by Fogarty International Fellowship FSTW02910A to A.C. who was on leave from the Department of Neurosurgery, University of Virginia. We thank Ann Lohr and Lee Snavely for technical assistance.

    Permanent address: Instituto de Investigacion Medica Mercedes y Martin Ferreyra, Casilla de Correo 389, 5000 Cordoba, Argentina.

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