Short communicationMAP2 is localized to the dendrites of hippocampal neurons which develop in culture☆
References (26)
- et al.
Rat hippocampal neurons in dispersed cell culture
Brain Res.
(1977) - et al.
Intracellular horseradish peroxidase injection for correlation of light and electron microscopic anatomy with synaptic physiology of cultured mouse spinal cord neurons
Brain Res.
(1978) Trophic interactions between astroglial cells and hippocampal neurons in culture
Science
(1980)- et al.
Further observations on hippocampal neurons in dispersed cell culture
J. comp. Neurol.
(1979) - Bartlett, W. B. and Banker, G. A., An electron microscopic study of the development of axons and dendrites by...
- Bartlett, W. B. and Banker, G. A., An electron microscopic study of the development of axons and dendrites by...
- et al.
Initial phase of dendritic growth: evidence for the involvement of high molecular weight microtubule-associated proteins (HMWP) before the appearance of tubulin
J. Cell Biol.
(1982) Cellular differentiation: development of dendritic arborizations under normal and experimentally altered conditions
Neurosci. Res. Prog. Bull.
(1982)- et al.
Production and analysis of monoclonal antibodies specific for β-tubulin and MAP2
J. Cell Biol.
(1982) - et al.
Association of microtubule-associated protein (MAP2) with microtubules and intermediate filaments in cultured brain cells
J. Cell Biol.
(1983)
Growth of a rat neuroblastoma cell line in serum-free supplemented medium
Differential subcellular localization of tubulin and the microtubule associated protein MAP2 in brain tissue as revealed by immunocytochemistry with monoclonal hybridoma antibodies
J. Neurosci.
Immunocytochemical localization of actin and microtubule associated protein (MAP2) in dendritic spines
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Phase separation on microtubules: from droplet formation to cellular function?
2024, Trends in Cell BiologyCypin binds to tubulin heterodimers and microtubule protofilaments and regulates microtubule spacing in developing hippocampal neurons
2022, Molecular and Cellular NeuroscienceNeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
2021, Journal of Neuroscience MethodsCitation Excerpt :For example, NeuriteNet would be well-suited for establishing molecular mechanisms underlying morphological distinctions between dendrites and axons. In cultures of hippocampal neurons, this could be accomplished by double-labeling for proteins that selectively mark dendrites or axons (e.g., MAP2 (Caceres et al., 1984) and tau (Binder et al., 1985)). In addition, NeuriteNet could be adapted for high-throughput screening assays to identify compounds that may affect neurite growth or be neuroprotective (Li et al., 2021), or to screen for the genetic underpinnings of neuropsychological or neurodevelopmental disorders which may be linked to alterations in neuronal morphology (Krey et al., 2013; Yamashiro et al., 2020).
Nrg1/ErbB2 regulates differentiation and apoptosis of neural stem cells in the cochlear nucleus through PI3K/Akt pathway
2021, Neuroscience LettersCitation Excerpt :Nestin is a member of the family of intermediate filaments and the NSCs marker protein. Microtubule associated protein 2 (MAP2) is regarded as a somatodendritic marker for all dendrites and the proximal part of axons [10]. Glial fibrillary acidic protein (GFAP) is a monomeric intermediate filament protein and expressed in the astroglial skeleton, which was regarded as a marker of astrocyte.
Protocol for Evaluating Neuronal Polarity in Murine Models
2020, STAR Protocols
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This work was supported by NIH Grants NS17112 to G.B.) and N.S12333 (to O.S.) and by Fogarty International Fellowship FSTW02910A to A.C. who was on leave from the Department of Neurosurgery, University of Virginia. We thank Ann Lohr and Lee Snavely for technical assistance.
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Permanent address: Instituto de Investigacion Medica Mercedes y Martin Ferreyra, Casilla de Correo 389, 5000 Cordoba, Argentina.