Epitope mapping of monoclonal antibodies to Torpedo acetylcholine receptor γ subunits, which specifically recognize the ϵ subunit of mammalian muscle acetylcholine receptor

https://doi.org/10.1016/0165-5728(92)90027-IGet rights and content

Abstract

Epitopes for four monoclonal antibodies (mAbs) to the γ subunit of Torpedo nicotinic acetylcholine receptor (AChR), and one mAb crossreactive with the γ and δ subunits of Torpedo AChR were mapped using overlapping synthetic peptides corresponding to the complete amino acid sequence of Torpedo γ subunit. The epitopes for all mAbs were within a 50 residue sequence region, on the cytoplasmic surface of the AChr. Three mAbs crossreacted with mammalian muscle AChRs. Two of them specifically recognized the ∈ subunit of AChRs at adult neuromuscular junction.

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    Current address: Comparative Neuromuscular Laboratory, Off. of Vet. Services, M-104, University of California, San Diego, CA 92093, USA.

    ∗∗

    Current address: University of Pennsylvania Medial School, Institute of Neurological Sciences, 215 Medical Education Building, Philadelphia, PA 19104-6074, USA

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