Research reportEarly posttranslational modifications of the three neurofilament subunits in mouse retinal ganglion cells: neuronal sites and time course in relation to subunit polymerization and axonal transport
References (50)
- et al.
The structure, biochemical properties, and immunogenicity of neurofilament peripheral regions are determined by phosphorylation state
J. Biol. Chem.
(1985) Isolation of neurofilament proteins and of immunologically active neurofilament degradation products from extracts of brain, spinal cord and sciatic nerve
Biochim. Biophys. Acta
(1981)- et al.
Immunological study of a neurofilament protein variant (S150) with polyclonal and monoclonal antibodies
Biochim. Biophys. Acta
(1987) - et al.
Phosphate content of mammalian neurofilaments
J. Biol. Chem.
(1982) - et al.
Multiple phosphorylation sites in mammalian neurofilament polypeptides
J. Biol. Chem.
(1982) - et al.
Characteristics of the protein kinase activity associated with rat neurofilament preparations
Biochim. Biophys. Acta.
(1983) - et al.
SDS-PAGE strongly overestimates the molecular masses of the neurofilament proteins
FEBS Lett.
(1984) Protein degradation in the mouse visual system I. Degradation of axonally transported and retinal proteins
Brain Res.
(1980)- et al.
Limited proteolytic modification of a neurofilament protein involves a proteinase activated by endogenous levels of calcium
Brain Res.
(1983) - et al.
Differential turnover of phosphate groups on neurofilament subunits in mammalian neurons in vivo
J. Biol. Chem.
(1986)
Characterization of a cyclic nucleotide- and calcium-independent neurofilament protein kinase activity in axoplasm from the squid giant axon
J. Biol. Chem.
Soluble, phosphorylated forms of the high molecular weight neurofilament protein in perikarya of cultured neuronal cells
Neurosci. Lett.
Neurofilament protein phosphorylation
In vivo phosphorylation of distinct domains of the 70-kilodalton neurofilament subunit involves different protein kinases
J. Biol. Chem.
Phosphorylation of neurofilament proteins by protein kinase C
FEBS Lett.
Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants
Nature (Lond.)
Light dependent antibody labelling of photoreceptors
Nature (Lond.)
Slow posttranslational modification of a neurofilament protein
J. Cell Biol.
Interval between the synthesis and assembly of cytoskeletal proteins in cultured neurons
J. Neurosci.
Slow components of axonal transport: two cytoskeletal networks
J. Cell Biol.
Characterization and comparison of neurofilament proteins from rat and mouse CNS
J. Neurochem.
Bulk preparation of CNS cytoskeleton and the separation of individual neurofilament proteins by gel filtration: Dye-binding characteristics and amino acid compositions
J. Neurochem.
Preparation of antisera to neurofilament protein from chicken brain and human sciatic nerve
J. Comp. Neurol.
Masking of epitopes in tissue sections
Antibodies to heavy neurofilament subunit detect a subpopulation of damaged ganglion cells in retina
Nature (Lond.)
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Atypical Expression and Activation of GluN2A- and GluN2B-Containing NMDA Receptors at Ganglion Cells during Retinal Degeneration
2018, NeuroscienceCitation Excerpt :Next, to provide further anatomical support for the functional change observed in the physiological data, we immunolabeled wt and rd10 retinal whole mounts with an antibody that recognizes the GluN2A-NMDAR subunit (n = 3 mice each). To insure that the differences in GluN2A expression are specific to ON α-RGCs, we utilized SMI-32, a neurofilament antibody that has been shown to predominantly label α-RGCs (Coombs et al., 2006; Lin et al., 2004; Nixon et al., 1989). Since many RGC subtypes exist, this approach allows for the comparison of the same cell type across animal models.
Transcription factors SOX4 and SOX11 function redundantly to regulate the development of mouse retinal ganglion cells
2013, Journal of Biological ChemistryCitation Excerpt :Quantification of BRN3B+ exhibited ∼21 and 28% reduction of RGCs in Sox4-nulls and Sox11-nulls, respectively (Fig. 3, P and Q). To confirm the loss of RGCs in mutants, SMI32, which predominantly marks large RGCs and their nerve fibers (31), was used as another parameter of RGC number. Immunostaining of whole mount retinas with SMI32 revealed that not only Sox4- or Sox11-null retinas had a obviously less number of axon bundles but also their axon bundles was less fasciculated (Fig. 3, D–D′, I–I′).
Does elevated intraocular pressure reduce retinal TRKB-mediated survival signaling in experimental glaucoma?
2009, Experimental Eye ResearchCitation Excerpt :We quantified mRNA levels of neurofilament H (Nefh) and Brn3 POU domain genes including Brn3a (Pou4f1), Brn3b (Pou4f2), and Brn3c (Pou4f3). Neurofilament mRNA is produced almost exclusively by RGC and NEFH protein is localized to their axonal neurofilaments (Nixon et al., 1989; McKerracher et al., 1993). Brn3a, b, and c are transcription factors specifically localized to RGC nuclei (Xiang et al., 1995).
Role of phosphorylation on the structural dynamics and function of types III and IV intermediate filaments
2007, Experimental Cell ResearchMultiple requirements for Hes1 during early eye formation
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