Expression of the gene encoding the β-subunit of S-100 protein in the developing rat brain analyzed by in situ hybridization

doi:10.1016/0169-328X(89)90071-5

Molecular Brain Research

Volume 6, Issue 4, December 1989, Pages 251-262

https://doi.org/10.1016/0169-328X(89)90071-5 Get rights and content

Abstract

To investigate patterns of expression of the gene encoding the β-subunit of S-100 protein during development of the rat brain we have used Northern blotting and in situ hybridization histochemistry. During late prenatal development β-S-100 mRNA was observed first in the germinal zone lining the 4th ventricle. In the postnatal cerebellum this mRNA accumulated primarily in Bergmann glia and astrocytes of the deep white matter. In the hindbrain, expression of S-100 mRNA increased steadily in specific regions during the first postnatal week while levels remained low in more anterior brain regions. By the end of the second postnatal week, a dense punctate signal was distributed throughout the midbrain and hindbrain. Expression in forebrain, first observed at E18, was confined to cells lining the ventricle until the second postnatal week when accumulation of mRNA was observed in specific regions of the hippocampus, neocortex and olfactory bulb. The adult brain pattern of β-S-100 mRNA distribution is attained during the third postnatal week. These results demonstrate a caudal-rostral gradient in expression of the β-S-100 gene during rat brain development, as well as pronounced regional differences which may reflect the differentiation of subpopulations of astrocytes.

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Research reportExpression of the gene encoding the β-subunit of S-100 protein in the developing rat brain analyzed by in situ hybridization

Abstract

Dev. Biol.

Neuroscience

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

Anal. Biochem.

Biochim. Biophys. Acta

TIBS

J. Mol. Biol.

Biochem. Biophys. Res. Commun.

Biochimie

Biochim. Biophys. Acta

Biochem. Biophys. Res. Commun.

J. Biol. Chem.

Dev. Biol.

J. Biol. Chem.

Gene encoding the β subunit of S100 protein is on chromosome 21: Implications for Down syndrome

Science

Differentiated rat glial cell strain in tissue culture

Science

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

Biochemistry

Synthesis of S100 protein on free and membrane-bound polysomes of the rabbit brain

J. Neurochem.

p1B15: a cDNA clone of the rat mRNA encoding cyclophilin

DNA

Presumptive common precursor for neuronal and glial cell lineages in mouse hypothalamus

Research report
Expression of the gene encoding the β-subunit of S-100 protein in the developing rat brain analyzed by in situ hybridization