The pattern of GAP-43 immunostaining changes in the rat hippocampal formation during reactive synaptogenesis

doi:10.1016/0169-328X(90)90004-W

Molecular Brain Research

Volume 8, Issue 1, June 1990, Pages 17-23

https://doi.org/10.1016/0169-328X(90)90004-W Get rights and content

Abstract

The reactive synaptogenesis that takes place in the rat hippocampal formation after certain experimental manipulations affords an opportunity to investigate the molecular events that underlie structural remodeling in the adult CNS. Between 2 and 4 days after lesioning the perforant pathway, levels of the synaptic phosphoprotein, GAP-43 (B50, F1, pp46, neuromodulin), were found to increase markedly in the inner molecular layer (iml) of the dentate gyrus, coincident with the time at which commissural-associational (CA) fibers begin to sprout axon collaterals into dendritic portions denervated by the lesion. GAP-43 immunostaining in the iml began to decline by 8 days but continued to define an expanded CA projection for at least one month. In the outer molecular layer (oml), GAP-43 levels decreased after the loss of perforant pathway terminals and did not return for 2–3 weeks, the time at which sprouting of septal inputs into this layer can be visualized by cholinesterase histochemistry. These results demonstrate that GAP-43 levels change during reactive synaptogenesis, and point to differences among neural systems in their expression of this protein.

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Research reportThe pattern of GAP-43 immunostaining changes in the rat hippocampal formation during reactive synaptogenesis

Abstract

Protein kinase C phosphorylates a 47 Mr protein directly related to synaptic plasticity

Brain Res.

Regulation of calmodulin binding to P-57

J. Biol. Chem.

Phosphorylation of B-50 protein by calcium-activated, phospholipid-dependent protein kinase and B-50 protein kinase

J. Neurochem.

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Hippocampal region

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Brain Res.

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J. Neurosci.

Increased transport of 44,000- to 49,000-Dalton acidic proteins during regeneration of the goldfish optic nerve: a two-dimensional gel analysis

J. Neurosci.

A membrane phosphoprotein associated with neural development, axonal regeneration, phospholipid metabolism, and synaptic plasticity

Trends Neurosci.

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J. Neurosci.

Synaptic plasticity in the mature CNS involves increased levels of GAP-43

Soc. Neurosci. Abstr.

Localization of the growth-associated phosphoprotein GAP-43 in the human cerebral cortex

J. Neurosci.

Prolonged alteration in composition of fast transported protein in axons prevented from regenerating after injury

J. Neurobiol.

Dependence of GAP-43 (B50, F1) transport on axonal regeneration in rat dorsal root ganglion neurons

Brain Res.

An immunohistochemical and biochemical study of the microtubule-associated protein MAP2 during post-lesion dendritic remodeling in the central nervous system of adult rats

Mol. Brain Res.

Synaptic rearrangement in the dentate gyrus: histochemical evidence of adjustments after lesions in immature and adult rats

Cell biology of synaptic plasticity

Science

Phosphoprotein B-50 in nerve growth cones from fetal rat brain

Neurosci. Lett.

Inhibition of noradrenaline release by antibodies to B-50 (GAP-43)

Nature (Lond.)

The building of the brain (neurogenesis): slow plastic changes and learning

Degeneration and regeneration in the outer molecular layer of the dentate gyrus after perforant path lesions: role of astrocytes, microglia and interleukin-I

Soc. Neurosci. Abstr.

Astrocytes are important for sprouting in the septohippocampal circuit

Exp. Neurol.

In situ hybridization of tubulin α-1 mRNA as a marker of neurons participating in reactive synaptogenesis

Soc. Neurosci. Abstr.

Development of neuronal polarity: GAP-43 distinguishes axonal from dendritic growth cones

Nature (Lond.)

The anatomy of a memory: convergence of results across a diversity of tests

Trends Neurosci.

A protein associated with axon growth, GAP-43, is widely distributed and developmentally regulated in rat CNS

J. Neurosci.

Modulation of brain polyphosphoinositide metabolism by ACTH-sensitive protein phosphorylation

Nature (Lond.)

A ‘direct-coloring’ thiocholine method for cholinesterases

J. Histochem. Cytochem.

Nerve growth cones isolated from fetal rat brain. III. Calcium-dependent protein phosphorylation

J. Neurosci.

Molecular cloning of cDNAs for the nerve-cell specific phosphoprotein, synapsin I

EMBO J.

A selective increase in phosphorylation of protein F1, a protein kinase C substrate, directly related to three day growth of long term synaptic enhancement

Brain Res.

Research report
The pattern of GAP-43 immunostaining changes in the rat hippocampal formation during reactive synaptogenesis

Protein kinase C phosphorylates a 47 M_r protein directly related to synaptic plasticity