Positive- and negative-acting promoter sequences regulate cell type-specific expression of the rat synapsin I gene

doi:10.1016/0169-328X(91)90044-X

Molecular Brain Research

Volume 11, Issues 3–4, October 1991, Pages 345-353

https://doi.org/10.1016/0169-328X(91)90044-X Get rights and content

Abstract

The phosphoprotein synapsin I is expressed exclusively in neuronal cells. We interested in elucidating the promoter sequences involved in cell type-specific expression of the synapsin I gene. The PC12 cell line expresses the 3.4 kb and 4.5 kb synapsin I mRNAs and is used to analyze cell typespecific gene expression. A series of deletion fragments of the rat synapsin I gene promoter were fused to the promoterless reporter gene encoding bacterial chloramphenicol acetyltransferase (CAT) for transfection analysis in PC12 cells and in HeLa cells, which do not express the gene. A −349 bp to +110 bp rat synapsin I promoter fragment contains a positive regulator, shown to be 33-times more active in PC12 cells than HeLa cells. Transfection of reporter plasmids containing up to 4.4. kb of rat synapsin I gene promoter sequences exhibit significantly reduced CAT activity in PC12 cells. The reduction in CAT expression was attributed to a negative regulator located between −349 bp and −1341 bp in the rat synapsin I promoter. Our results suggest that both positive and negative-acting sequence elements regulate cell type-specific expression of the rat synapsin I gene.

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^∗: Current address: Cephalon Inc., 145 Brandywine Pkwy, West Chester, PA 19380, U.S.A.

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Research reportPositive- and negative-acting promoter sequences regulate cell type-specific expression of the rat synapsin I gene

Abstract

Anal. Biochem.

Methods Enzymol.

Virology

Mol. Brain Res.

J. Biol. Chem.

Neuron

Neuron

J. Biol. Chem.

J. Biol. Chem.

Anal. Biochem.

Cell

J. Mol. Biol.

J. Biol. Chem.

Cell

Neuron

Gene

Neurotransmitter synthesis by neuroblastoma clones

Characterization of synapsin I fragments by cysteine-specific cleavage; a study of their interactions with F-actin

J. Cell Biol.

The synapsins and the regulation of synaptic function

Bioessays

Synapsin I bundles F-actin in a phosphorylation-dependent manner

Nature

Interactions of synapsin I with small synaptic vesicles: distinct sites in synapsin I bind to vesicle phospholipids and vesicle proteins

J. Cell Biol.

Structure of the NGFI-A gene and detection of upstream sequences responsible for its transcriptional induction by nerve growth factor

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

Biochemistry

Research report
Positive- and negative-acting promoter sequences regulate cell type-specific expression of the rat synapsin I gene