Elsevier

Molecular Brain Research

Volume 33, Issue 1, October 1995, Pages 97-103
Molecular Brain Research

Regional quantification of D1, D2, and D3 dopamine receptor mRNA in rat brain using a ribonuclease protection assay

https://doi.org/10.1016/0169-328X(95)00112-6Get rights and content

Abstract

We describe ribonuclease protection assays for dopamine D1, D2, and D3 receptors, and regional quantitation of mRNA levels for these receptors in rat brain. Both D1 and D2 mRNA levels were highest in caudate putamen, where they were found in approximately equal levels. Of the brain regions examined, D3 mRNA was most abundant in hippocampus, hypothalamus, and nucleus accumbens. Levels of D3 mRNA were significantly lower than values for D1 and D2 mRNA in all brain regions studied. Variability was observed between animals for expression of both D1 and D2 mRNA in caudate putamen, with a significant correlation between D1 and D2 mRNA levels in neostriatum (r = 0.942, P < 0.001). This suggests a functional interaction between D1 and D2 receptor mRNA levels in this brain region. Our results are generally consistent with regional distributions previously reported using other methods. These results suggest that DA D2 receptors function both as an auto and as a postsynaptic receptor, while D1 receptors are restricted to a postsynaptic function. Our results demonstrate the utility of this method in studying possible relationships between individual animal variation in regional mRNA expression and behavioral response to pharmacological and other experimental treatments.

References (41)

  • SnyderL.A. et al.

    Distribution of dopamine D2 receptor mRNA splice variants in the rat by solution hybridization/protection assay

    Neurosci. Lett.

    (1991)
  • SokoloffP. et al.

    The third dopamine receptor (D3) as a novel target for antipsychotics

    Biochem. Pharmacol.

    (1992)
  • StoofJ.C. et al.

    Stimulation of D2-dopamine receptors in rat neostriatum inhibits the release of acetylcholine and dopamine but does not affect the release of gamma-aminobutyric acid, glutamate or serotonin

    Eur. J. Pharmacol.

    (1982)
  • StrangeP.G.

    New insights into dopamine receptors in the central nervous system

    Neurochem. Int.

    (1993)
  • Van TolH.H. et al.

    Lack of effect of chronic dopamine receptor blockade on D2 dopamine receptor mRNA level

    Neurosci. Lett.

    (1990)
  • BrewerG. et al.

    RNase ONE Advantages for nuclease protection assays

    Promega Notes

    (1992)
  • BunzowJ.R. et al.

    Cloning and expression of a rat D2 dopamine receptor cDNA [see comments]

    Nature

    (1988)
  • CivelliO. et al.

    Molecular diversity of the dopamine receptors

    Annu. Rev. Pharmacol. Toxicol.

    (1993)
  • DanielsonP.E. et al.

    p1B15: a cDNA clone of the rat mRNA encoding cyclophilin

    DNA

    (1988)
  • DearryA. et al.

    Molecular cloning and expression of the gene for a human D1 dopamine receptor

    Nature

    (1990)
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    Present adress: Cincinnati Veterans Affairs Medical Center, Department of Psychiatry, V-116A, 3200 Vine Street, Cincinnati, OH 45220, USA and Department of Psychiatry, University of Cincinnati College of Medicine, 231 Bethesda Avenue (ML 559), Cincinnati, OH 45267-0559, USA.

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