Localization of the Thy-1 antigen to the surfaces of rat retinal ganglion cells

doi:10.1016/0197-0186(82)90049-3

Neurochemistry International

Volume 4, Issue 6, 1982, Pages 587-595

https://doi.org/10.1016/0197-0186(82)90049-3 Get rights and content

Abstract

Indirect immunofluorescence has been used to localize the Thy-1 antigen to ganglion cell axons, the ganglion cell layer and the inner plexiform layer in cryostat sections of adult and neonatal rat retina. In similar immunofluorescence experiments monoclonal antibodies raised against the 200,000 molecular weight neurofilament polypeptide bound only to ganglion cell axons and processes in the outer plexiform layer.

Less than 1% of cells dissociated from 8 day postnatal rat retina had superficial Thy-1 antigen demonstrable by immunofluorescence; these cells were generally large and their size spectrum was similar to that of ganglion cells $in$ $situ$ . After culture for 1 day many of these Thy-1 positive cells had generated neurofilament antigen.

We conclude that Thy-1 is found chiefly or exclusively on ganglion cells of eight day retina, and may be useful in the identification and isolation of these cells by immunoselection procedures.

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However, Thy-1 is differentially expressed in the different types of neurons. For example, neurons with long processes, such as the RG cells, express Thy-1 at higher density than those with short processes, like the interneurons (Barnstable and Drager, 1984; Beale and Osborne, 1982; Morris et al., 1985; Weber et al., 1988). Thy-1 expression is also regulated during pre- and postnatal development.
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The levels of Thy-1, neurofilament light (NF-L; MW 70 to 80 kd) and internal standard, 18S ribosomal RNA (rRNA), from total RNAs present in the retinas were determined with a comparative quantitative RT-PCR technique (Ambion Inc., Austin TX). Thy-1 antigen is primarily associated with retinal ganglion cells.32 NF-L is primarily associated with ganglion cell axons.33
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Rapid paperLocalization of the Thy-1 antigen to the surfaces of rat retinal ganglion cells

Abstract

J. Neurosci. Methods

Neurosci.

J. Neuroimmunol.

J. Neuroimmunol.

Dev. Biol.

Neurofibrillary tangles and neurofilaments cross-react with monoclonal antibodies

Biol. Cell

Localization of the Thy-1 antigen in the cerebellar cortex of rat brain by immunofluorescence during postnatal development

J. Neurochem.

Localization of the Thy-1 antigen in rat brain and spinal cord by immunofluorescence

J. Neurochem.

Localization of the Thy-1 antigen by immunofluorescence on neurons isolated from rat brain

J. Neurochem.

Monoclonal antibodies which recognise different cell types in rat retina

Nature

Rat neural antigen-2 (Ran-2): a cell surface antigen on astrocytes, ependymal cells, Müller cells and lepto-meninges defined by a monoclonal antibody

Brain Res.

The characterisation of cell cultures of neonatal retina

The binding of tetanus toxin to retina cells

Brain Res.

Structural similarities between Thy-1 antigen from rat brain and immunoglobulin

Nature

A neuronal cell-surface antigen is found in the CNS but not in peripheral neurones

Nature

Rapid paper
Localization of the Thy-1 antigen to the surfaces of rat retinal ganglion cells