Accumulation of β-Amyloid fibrils in pancreas of transgenic mice
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Attenuation of acetaldehyde-induced cell injury by overexpression of aldehyde dehydrogenase-2 (ALDH2) transgene in human cardiac myocytes: Role of MAP kinase signaling
2006, Journal of Molecular and Cellular CardiologyCitation Excerpt :Intracellular oxidative stress was monitored by microscopic fluorescence intensity changes of CM-H2DCFDA [20] or by intracellular fluorescence detection using a Spectra MaxGeminiXS device (Spectra Max, Atlanta, GA, USA) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm [21]. For microscopic assay, cells were loaded with CM-H2DCFDA (1 μM) for 30 min at 37 °C before being evaluated using an Olympus IX-70 microscope and ImagePro image software [17]. Fluorescence was calibrated with InSpeck microspheres (Molecular Probes).
Reduced β-amyloid production and increased inflammatory responses in presenilin conditional knock-out mice
2004, Journal of Biological ChemistryCitation Excerpt :Same amounts of protein were resolved by SDS-PAGE electrophoresis and transferred to polyvinylidene difluoride membranes as described (10). For Fig. 1A, blots were incubated with the Saeko antiserum (13) followed by 125I-labeled anti-rabbit antibody (ICN Biomedicals). For Fig. 2B, blots were incubated with antibodies against glial fibrillary acidic protein (GFAP) (1:100,000; Sigma), cathepsin S (1:1000; kind gift of Guo-Ping Shi, Boston, MA), or complement component C1q (1:1000; Quidel) and developed with enhanced chemiluminescence (PerkinElmer Life Sciences).
Overexpression of aldehyde dehydrogenase-2 (ALDH2) transgene prevents acetaldehyde-induced cell injury in human umbilical vein endothelial cells: Role of ERK and p38 mitogen-activated protein kinase
2004, Journal of Biological ChemistryCitation Excerpt :Generation of the ALDH2 Transgene Construct and Its Expression in HUVECs—The human ALDH2 gene was amplified by PCR from pT7–7-hpALDH2 (provided by Dr. Henry Weiner from Purdue University, Lafayette, IN) using the following primers: ALDH-F (5′-tcgaattctatgttgcgcgctgccgcccg) and ALDH-R (3′-cacggagtcttcttgagtattcttaaggc). The amplified ALDH2 fragment was digested with EcoRI and cloned into the EcoRI site of vector pBsCAG-2 under the CAG cassette, which was shown to drive expression in many mammalian tissues (15). The transgene construct was designated as pCAG-ALDH2 (Fig. 1).