Analysis of phosphorylation of tau with antibodies specific for phosphorylation sites
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2008, NeuroscienceCitation Excerpt :Sections were incubated at room temperature with 0.01% Triton X-100 in PBS for 30 min and for another hour in 3% bovine serum albumin (BSA) in PBS. For immunolabeling, sections were incubated with antibodies to β-amyloid 1-40/42 (Alpha Diagnostic International), eNOS (Sigma Chemical), poly (ADP-ribose) polymerase (PARP) p85 (Promega, Madison, WI, USA), phospho tau 404 (Ishiguro et al., 1995), and phospho-GSK-3α/β-Ser279/216 (Biosource International) overnight at 4 °C. After washing, sections were incubated with biotinylated anti-rabbit IgG (1:5000) in TNB buffer for 1 h, followed by 2 h incubation with streptavidin–HRP (1:5000) and Alexa 594 anti-mouse IgG (Molecular Probes, Eugene, OR, USA) in TNB buffer (1:400).
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