Cortical projections arising from the basal forebrain: A study of cholinergic and noncholinergic components employing combined retrograde tracing and immunohistochemical localization of choline acetyltransferase

doi:10.1016/0306-4522(84)90083-6

Neuroscience

Volume 13, Issue 3, November 1984, Pages 627-643

https://doi.org/10.1016/0306-4522(84)90083-6 Get rights and content

Abstract

The neurochemical identity of ascending putative cholinergic pathways from the rat basal forebrain was investigated employing a method for simultaneouly visualizing choline acetyltransferase immunoreactivity and retrogradely transported horseradish peroxidase-conjugated wheatgerm agglutinin. This histochemical procedure revealed three distinct populations of neurons: (1) cells which stained only for choline acetyltransferase immunoreactivity; (2) cells which stained only for retrograde tracer and (3) cells which stained simultaneously for choline acetyltransferase immunoreactivity and retrograde tracer. The results demonstrated that this projection is topographically organized and consists of both cholinergic and noncholinergic components. The relative contribution of each component varied with the telencephalic target area as follows: the olfactory bulb receives a projection from cells of the horizontal limb nucleus, 10–20% of which are cholinergic (Ch3); the hippocampal formation receives afferents from cells of the medial septal and vertical limb nuclei, 35–45% of which are cholinergic (Chl and Ch2); and the cortical mantle receives afferents primarily from cells within the substantia innominata-nucleus basalis complex, 80–90% of which are cholinergic (Ch4). The topographical organization of Ch4 projections is not as completely differentiated as we have previously observed in the primate.

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Cortical projections arising from the basal forebrain: A study of cholinergic and noncholinergic components employing combined retrograde tracing and immunohistochemical localization of choline acetyltransferase

Abstract

Brain Res.

Brain Res. Bull.

Brain Res.

Neuroscience

Neuroscience

Brain Res. Rev.

neuroscience

Expl Neurol.

Brain Res.

Neuroscience

Brain Res.

Neuroscience

Brain Res.

Brain Res.

Brain Res.

Brain Res.

Brain Res. Bull.

Expl Neurol.

Brain Res.

Brain res. Bull.

Brain Res.

Neurochem. Int.

Brain Res. Rev.

Neuroscience

Brain Res. Bull.

Neurosci. Lett.

Neuroscience

Distribution of cholinergic neurons in the rat brain demonstrated by the immunohistochemical localization of choline acetyltransferase

J. comp. Neurol.

Differential projections of the basal forebrain and nuclei of the subthalamus to visual cortex in the rat

Soc. Neurosci. Abstr.

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Soc. Neurosci. Abstr.

Projections of the interstitial nucleus surrounding the globus pallidus: a study of retrograde changes following cortical ablations in rabbits

Z. Anal. EntwGesch.

The afferent connections of the main and the accessory olfactory bulb formations in the rat: an experimental HRP study

J. comp. Neurol.

Choline acetyltransferase and acetylcholinesterase containing projections from the basal forebrain to the amygdaloid complex of the rat

Brain Res.

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J. comp. neurol.

Studies on the comparative anatomy of the ganglion basale of Meynert

Acta anat.

The diencephalon of the albino rat

J. comp. Neurol.