Independent cellular and ontogenetic expression of mRNAS encoding three α polypeptides of the rat GABAA receptor

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Abstract

Previous studies have shown that several distinct but related polypeptides can serve as α subunits of functional GABAA receptors. Furthermore, the diversity of these polypeptides at least partially accounts for the functional heterogeneity of GABAA receptors. In this paper, we report the results of in situ hybridization studies using probes derived from our recently reported cDNAs for α1, α2, and α4 GABAA receptor polypeptides. We show that the mRNAs that encode these isoforms have distinct regional and cellular distributions and are present at widely varying levels within the rat brain. In addition, our Northern blot analyses indicate that each of these three α mRNAs has a distinct pattern of ontogenetic regulation.

Differential regulation of a polypeptide isoforms may lead to changes in GABAA receptor function during ontogeny as well as to distinct cellular responses to GABA and GABA-related drugs.

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    *

    Present address: Department of Neuroscience, University of Florida College of Medicine, Box J-244, J.H. Miller Health Center, Gainesville, FL 32610, U.S.A.

    ‡‡

    Present address: UnitéINSERm 29, 123 Boulevard de Port Royal, 75014 Paris, France.

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