Whole cell currents and mechanical responses of isolated outer hair cells
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2022, iScienceCitation Excerpt :Mechanosensitive ion channels (MSCs) convert mechanical stimulus to electrical or chemical activity. Native mechanical stimulation occurs in form of touch and pressure sensation (Santos-Sacchi and Dilger, 1988), changes in osmotic pressure, stress due to the blood flow, and bone structure. MSCs are essential for the development of tissues and organs (Soattin et al., 2016), as well as the differentiation of neuronal stem cells(Pathak et al., 2014).
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2020, Cell ReportsCitation Excerpt :The hair bundles were deflected using stiff glass probes mounted on a mechanical piezo actuator (Physik Instrument, USA) and filtered with an 8-pole Bessel filter at 50 kHz (NPI electronic, LPBF-01GX, Germany) to eliminate residual pipette resonance(Stauffer and Holt, 2007). The glass probes were polished to fit into the concave aspect of the array of the OHC stereocilia (Fettiplace and Kim, 2014; Santos-Sacchi and Dilger, 1988). For electrophysiological recording of the Ca2+-activated K+ currents of OHCs, the apical organ of Corti were separated rapidly from cochleae of P13∼14 mice on ice and digested by Collagenase IV (100 U/μL in HBSS with Ca2+ and Mg2+, 17104019, GIBCO) at 37°C, and then mechanically dissociated into single cell suspension.