Immunological, morphological, and electrophysiological variation among retinal ganglion cells purified by panning

doi:10.1016/0896-6273(88)90127-4

Neuron

Volume 1, Issue 9, November 1988, Pages 791-803

https://doi.org/10.1016/0896-6273(88)90127-4 Get rights and content

Abstract

Two different monoclonal antibodies to the Thy-1 antigen, T11D7 and 2G12, were used to purify and characterize retinal ganglion cells from postnatal rat retina. Although Thy-1 has been reported to be a specific marker for ganglion cells in retina, retinal cell suspensions contained several other types of Thy-1-positive cells as well. Nevertheless, a simple two-step “panning” procedure allowed isolation of ganglion cells to nearly 100% purity. We found that postnatal ganglion cells differed in antigenic, morphological, and intrinsic electrophysiological characteristics, and that these properties were correlated with one another. Minor variations of this panning protocol should allow rapid, high yield purification to homogeneity of many other neuronal and glial cell types.

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Neuron

Immunological, morphological, and electrophysiological variation among retinal ganglion cells purified by panning

Abstract

Neurosci. Lett.

Mol. Immunol.

Brain Res.

Neuroscience

Neurochem. Int.

Neurochem. Int.

Neurosci. Let.

Dev. Biol.

Brain Res.

Exp. Eye Res.

Dev. Brain Res.

Brain Res.

J. Immunol. Meth.

Dev. Brain Res.

J. Neurosci. Meth.

Pharmacological inhibition of the M-current

J. Physiol.

Variation of membrane properties in hair cells isolated from the turtle cochlea

J. Physiol.

Response to light of solitary rod photoreceptors isolated from tiger salamander retina

Purification and quantification of T and B lymphocytes by an affinity method

Immunology

Cell separation by velocity sedimentation of postnatal mouse cerebellum

J. Cell Physiol.

Ion channel phenotype of three glial cell types: a comparison of in vivo and in vitro channel expression

The morphological types of ganglion cells of the domestic cat's retina

J. Physiol.

Science and technology of patch-recording electrodes

The calcium current in inner segments of rods from the salamander (Ambystoma tigrinum) retina

J. Physiol.

Activity and the control of ganglion cell death in the rat retina