Neuron
Volume 6, Issue 6, June 1991, Pages 859-867
Journal home page for Neuron

Article
A diffusible second messenger mediates one of the pathways coupling receptors to calcium channels in rat sympathetic neurons

https://doi.org/10.1016/0896-6273(91)90226-PGet rights and content

Abstract

Muscarinic and α-adrenergic suppression of current through Ca2+ channels was studied in adult rat superior cervical ganglion neurons using whole-cell and cell-attached configurations of the patch-clamp technique. Oxotremorine methiodide suppressed Ica by both a rapid (<1 s) and a slow (>4 s) process, whereas norepinephrine suppressed Ica only by a rapid process. The slow muscarinic suppression could be prevented by adding 20 mM BAPTA, a Ca2+ chelator, to the recording pipette, whereas the adrenergic suppression was not affected. Muscarinic, but not α-adrenergic, receptors can couple to Ca2+ channels by a second messenger capable of diffusing into an on-cell patch. This signal seems not to be carried by intracellular Ca2+ cGMP, cAMP, or protein kinase C.

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    Present address: Department of Pharmacology, St. George's Hospital Medical School, Cranmer Terrace, London SW17 ORE, England.

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