The use of acetylated ferricytochrome C for the detection of superoxide radicals produced in biological membranes

doi:10.1016/S0006-291X(75)80188-4

Biochemical and Biophysical Research Communications

Volume 65, Issue 2, 22 July 1975, Pages 597-603

https://doi.org/10.1016/S0006-291X(75)80188-4 Get rights and content

Summary

Acetylation of 60% of lysine residues of horse heart ferricytochrome c results in more than 95% decrease of its ability to be reduced by mitochondrial and microsomal reductases and to become oxidized (after chemical reduction) by mitochondrial oxidase. The ability of acetylated ferricytochrome c to be reduced by 0₂⁻ radicals is maintained, making this derivative useful for the detection of 0₂⁻ radicals in biological systems containing cytochrome c reductases or oxidases. Mitochondrial membranes can reduce acetylated ferricytochrome c at a rate of 0.5 nmoles.min⁻¹.mg⁻¹. Such a reaction is 82% inhibited by 2.8 × 10⁻⁸M superoxide dismutase.

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The use of acetylated ferricytochrome C for the detection of superoxide radicals produced in biological membranes

Summary

J. Biol. Chem.

J. Biol. Chem.

Biochim. Biophys. Acta

Methods in Enzymol.

J. Biol. Chem.

Methods in Enzymol.

Methods in Enzymol.

FEBS Lett.